DKK1 active protein

DKK1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123257

• Gene Names: Dkk1; mdkk-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DKK1; DKK1 Protein; Mouse; Recombinant (His Tag); Mouse DKK-1 Protein (His Tag); mdkk-1 Protein; dickkopf WNT signaling pathway inhibitor 1; DKK1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His272

• Species: Mouse
• Activity: Measured by its ability to inhibit recombinant rmWnt3a induced alkaline phosphatase production by C3H10T 1/2 cells. The ED50 for this effect is 0.05-0.3ug/mL.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse DKK1 (NP_034181.2)(Met1-His272) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit recombinant rmWnt3a induced alkaline phosphatase production by C3H10T 1/2 cells. The ED50 for this effect is 0.05-0.3 ug/mL.)

Related Product Information for DKK1 active protein

Background: Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Categories/Family for DKK1 active protein

Canonical Wnt Pathway

References

Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.

NCBI and Uniprot Product Information

• NCBI GI #: 31542557
• NCBI GeneID: 13380
• NCBI Accession #: NP_034181.2
• NCBI GenBank Nucleotide #: NM_010051.3
• UniProt Accession #: O54908; Q80UL5
• Molecular Weight: 29,298 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: Dkk1
• NCBI Official Synonym Symbols: mdkk-1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: Dkk1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; mDkk-1
• UniProt Entry Name: DKK1_MOUSE

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: receptor antagonist activity; protein binding; low-density lipoprotein receptor binding

Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; Wnt receptor signaling pathway; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; multicellular organismal development; regulation of receptor internalization; regulation of Wnt receptor signaling pathway; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; hair follicle development; negative regulation of mesodermal cell fate specification; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; endoderm development; embryonic limb morphogenesis