Rabbit anti-Human, Mouse Dkk-1 Polyclonal Antibody | anti-DKK1 antibody

Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)

Cat. Num. AAA611235

• Gene Names: DKK1; SK; DKK-1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by epitope affinity chromatography.
• Synonyms: Dkk-1; Polyclonal Antibody; Dkk-1 (DKK1; SK; DKK-1; Dickkopf Homolog 1 (Xenopus laevis); Dickkopf-1 Like; Dickkopf (Xenopus laevis) Homolog 1); Anti -Dkk-1 (DKK1; anti-DKK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human and mouse Dkk-1 proteins. On Western blots, it identifies the target band at ~35kD. Reactivity has been confirmed with human Dkk-1-transfected 293T cell lysates.
• Purity/Purification: Affinity Purified; Purified by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-DKK1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.1-2ug/ml
• Immunogen: Synthetic peptide derived from the C-terminal region of the human and mouse Dkk-1(Dickkopf-1) proteins.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DKK1 antibody

The human Dickkopf (Dkk)-related proteins are a structurally and functionally heterogeneous family of proteins. This family is comprised of hDkk1, -2, -3, -4 and Soggy (Sgy), a Dkk-3 related protein. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues and are secreted when expressed in 293T cells. Several secreted antagonists, including Dickkopf-1 (Dkk-1) (German for "big head, stubborn") regulate the activity of Wnt proteins secreted glycoproteins that influence many aspects of embryonic development in metazoans. Dkk-1 is a head inducer secreted from the vertebrate head organizer, that induces anterior development of Xenopus by antagonizing Wnt signaling. Dkk-1 inhibits Wnt-induced axis duplication, acts upstream of disheveled3 and antagonizes Wnt signaling upstream of its effect on beta-catenin regulation. Dkk-1 is an LRP6 ligand5 that inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation. Dkk-1 plays a crucial role in the control of programmed cell death during vertebrate limb development. Bmp-4 is one of several upstream regulators of Dkk-1 expression. Furthermore, the proapoptotic hDkk-1 gene may play important roles in linking the oncogenic Wnt and p53 tumor suppressor pathways.

Product Categories/Family for anti-DKK1 antibody

Antibodies; Abs to Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374.1
• NCBI GenBank Nucleotide #: NM_012242.2
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28,672 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf homolog 1 (Xenopus laevis)
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1; hDkk-1; dickkopf-1 like; OTTHUMP00000019617; dickkopf related protein-1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK1
• UniProt Entry Name: DKK1_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq]

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; early endosome membrane; extracellular region; plasma membrane

Molecular Function: signal transducer activity; protein binding; receptor antagonist activity; growth factor activity; low-density lipoprotein receptor binding

Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; mesoderm formation; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis