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SDS-Page

Galectin 3 (GAL3) Recombinant Protein | GAL3 recombinant protein

Recombinant Galectin 3 (GAL3)

Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Galectin 3 (GAL3); Recombinant Galectin 3 (GAL3); GAL3 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
200ug/mL (varies by lot)
Applicable Applications for GAL3 recombinant protein
Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
Application Notes
May be suitable for use in other assays to be determined by the end user.
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Met1~Ile250
Tags
N-terminal His Tag
Subcellular Location
Nucleus, Secreted, Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
8.6
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

Gene Sequencing (extract)

Gene Sequencing (extract)

Identification

Identification

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 27.4kDa
Accurate Molecular Mass: 28 kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
galectin-3 isoform 1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 3
NCBI Official Symbol
LGALS3
NCBI Official Synonym Symbols
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
NCBI Protein Information
galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
UniProt Protein Name
Galectin-3
UniProt Gene Name
LGALS3
UniProt Synonym Gene Names
MAC2; Gal-3; CBP 35; GALBP
UniProt Entry Name
LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Cell surface

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: spliceosome; extracellular matrix; proteinaceous extracellular matrix; extracellular space; membrane; cytoplasm; mitochondrial inner membrane; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; negative regulation of endocytosis; RNA splicing; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

Research Articles on GAL3

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Product Notes

The GAL3 lgals3 (Catalog #AAA2009298) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Galectin 3 (GAL3) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB). May be suitable for use in other assays to be determined by the end user. Researchers should empirically determine the suitability of the GAL3 lgals3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin 3 (GAL3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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