Rabbit DJ-1 Polyclonal Antibody | anti-PARK7 antibody

DJ-1 Antibody (N-term)

Cat. Num. AAA9202523

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Zebrafish)
• Applications: Western Blot, ELISA, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DJ-1; Polyclonal Antibody; DJ-1 Antibody (N-term); Protein DJ-1; 34--; Oncogene DJ1; Parkinson disease protein 7; PARK7; anti-PARK7 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Zebrafish)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DJ-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DJ-1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-Park7 (DJ-1) Antibody (N-term) in mouse brain,kidney,liver tissue lysates and NIH-3T3, Hela cell line lysates (35ug/lane). Park7 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DJ-1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PARK7 antibody

Park 7 acts as positive regulator of androgen receptor-dependent transcription, and may function as redox-sensitive chaperone and as sensor for oxidative stress, as well as preventing aggregation of SNCA. This protein has been shown to protect neurons against oxidative stress and cell death, and to play a role in fertilization. Park7 is detected in tau inclusions in brains from neurodegenerative disease patients, and is generally highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, with detectable levels in placenta, brain, astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Defects in Park7 are the cause of autosomal recessive early-onset Parkinson disease 7 (PARK7), a form of Parkinson disease characterized by onset before 40 years, slow progression and initial good response to levodopa.

Product Categories/Family for anti-PARK7 antibody

Cancer; Cell Biology; Metabolism; Neuroscience; Signal Transduction

References

Kim, R.H., et al., Cancer Cell 7(3):263-273 (2005). Shinbo, Y., et al., Int. J. Oncol. 26(3):641-648 (2005). Takahashi-Niki, K., et al., Biochem. Biophys. Res. Commun. 320(2):389-397 (2004). Hering, R., et al., Hum. Mutat. 24(4):321-329 (2004). Maraganore, D.M., et al., Neurology 63(3):550-553 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1; NP_009193.2
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19891
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• Protein Family: DJ-1 protein
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Transcription regulation; Nuclear receptor co-regulator; Tumor suppressor; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; mitochondrial matrix; axon; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; chromatin; mitochondrial respiratory chain complex I; cytosol; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; mRNA binding; peptidase activity; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; lactate biosynthetic process; autophagy; negative regulation of protein kinase activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset