Rabbit Dishevelled 3 Polyclonal Antibody | anti-DVL3 antibody

Dishevelled 3 Polyclonal Antibody

Cat. Num. AAA3005827

• Gene Names: DVL3; DRS3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Dishevelled 3; Polyclonal Antibody; Dishevelled 3 Polyclonal Antibody; Segment polarity protein dishevelled homolog DVL-3; Dishevelled-3; Dishevelled3; DSH homolog 3; DVL3; KIAA0208; anti-DVL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Dishevelled 3 polyclonal antibody detects endogenous levels of Dishevelled 3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 716

• Applicable Applications for anti-DVL3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 368-413 of Human Dishevelled 3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Dishevelled 3 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:Rat kidney tissue lysate)

Related Product Information for anti-DVL3 antibody

Mammalian homologs of the Drosophila dishevelled (Dsh) gene have been identified, including Dvl-1, Dvl-2 and Dvl-3. The mammalian dishevelled proteins contain three homologous domains, two of which are unrelated to any other known protein. The third region is homologous to the discs-large homology domain of Drosophila discs-large-1, a tumor suppressor protein. Like their Drosophila counterpart, the dishevelled proteins are thought to be in-volved in embryogenesis. Overexpression of Dvl-1 has been shown to inhibit the phosphorylation of tau by GSK-3b. This finding may prove to be important in Alzheimer's studies, which have shown that tau is hyperphosphorylated. In Drosophila, Dsh is a component of the frizzled signaling pathway. Both mammalian dishevelled and frizzled proteins are components of the Wnt signalling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 41406097
• NCBI GeneID: 1857
• NCBI Accession #: NP_004414.3
• NCBI GenBank Nucleotide #: NP_004414.3
• UniProt Accession #: Q92997
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: segment polarity protein dishevelled homolog DVL-3
• NCBI Official Synonym Full Names: dishevelled segment polarity protein 3
• NCBI Official Symbol: DVL3
• NCBI Official Synonym Symbols: DRS3
• NCBI Protein Information: segment polarity protein dishevelled homolog DVL-3
• UniProt Protein Name: Segment polarity protein dishevelled homolog DVL-3
• Protein Family: Segment polarity protein dishevelled
• UniProt Gene Name: DVL3
• UniProt Synonym Gene Names: KIAA0208; Dishevelled-3
• UniProt Entry Name: DVL3_HUMAN

NCBI Description

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

DVL3: a protein in the WNT/planar cell polarity (PCP) signaling pathway. Dvl2-deficient mice indicate that Dvl2 is essential for normal cardiac morphogenesis, somite segmentation and neural tube closure. Transduces the Wnt signal by interacting with the cytoplasmic Axin complex. Dvl and Axin each contain a DIX domains, which mediate their dynamic polymerization. The Dvl-Axin interaction is essential for Wnt signaling. Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2. Interacts with Dixin and Rac. There is functional redundancy between Dvl1 and Dvl2 in some phenotypes.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: cytosol

Molecular Function: protein binding; protease binding; protein heterodimerization activity; frizzled binding; beta-catenin binding; receptor binding

Biological Process: response to drug; positive regulation of JNK activity; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation