Integrin Alpha 6 (ITGa6) Recombinant Protein | ITGa6 recombinant protein

Recombinant Integrin Alpha 6 (ITGa6)

Cat. Num. AAA2011863

• Gene Names: Itga6; Cd49f; VLA-6; AI115430; 5033401O05Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Integrin Alpha 6 (ITGa6); Recombinant Integrin Alpha 6 (ITGa6); ITGa6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-ELPEKQID DSRKFSLFPE RKYQTLNCSV NVRCVNIRCP LRGLDSKASL VLRSRLWNST FLEEYSKLNY LDILLRASID VTAAAQNIKL PHAGTQVRVT VFPSKTVAQY SGVAWWIILL AVLAGILMLA LLVFLLWKCG FFKRNKKDHY DATYHKAEIH TQPSDKERLT SDA
• Sequence Length: 1091

• Applicable Applications for ITGa6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu903~Ala1073 (Accession # Q61739) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 485836844
• NCBI GeneID: 16403
• NCBI Accession #: NP_001264899.1
• NCBI GenBank Nucleotide #: NM_001277970.1
• UniProt Accession #: Q61739; A2AU04
• Molecular Weight: 52.0kDa
• NCBI Official Full Name: integrin alpha-6 isoform 2
• NCBI Official Synonym Full Names: integrin alpha 6
• NCBI Official Symbol: Itga6
• NCBI Official Synonym Symbols: Cd49f; VLA-6; AI115430; 5033401O05Rik
• NCBI Protein Information: integrin alpha-6; CD49 antigen-like family member F
• UniProt Protein Name: Integrin alpha-6
• Protein Family: Integrin
• UniProt Gene Name: Itga6
• UniProt Entry Name: ITA6_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]

Uniprot Description

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Cellular Component: cell surface; focal adhesion; basolateral plasma membrane; integral to membrane; cell-cell adherens junction; hemidesmosome; membrane; plasma membrane; basal plasma membrane; basement membrane; integrin complex; filopodium; external side of plasma membrane

Molecular Function: integrin binding; metal ion binding; laminin binding; protein complex binding

Biological Process: integrin-mediated signaling pathway; skin development; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; odontogenesis of dentine-containing teeth; cell-cell adhesion; gut development; cellular response to extracellular stimulus; positive regulation of cell-cell adhesion; brown fat cell differentiation; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; leukocyte migration; positive regulation of phosphorylation