ARHI Recombinant Protein | DIRAS3 recombinant protein

ARHI Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121646

• Gene Names: DIRAS3; ARHI; NOEY2
• Purity: >85% as determined by SDS-PAGE
• Synonyms: ARHI; ARHI Protein; Human; Recombinant (mFc Tag); Human ARHI/DIRAS3 Protein (Fc Tag); DIRAS3 Protein; NOEY2 Protein; RHOI Protein; DIRAS family; GTP-binding RAS-like 3; DIRAS3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys225

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human DIRAS3 (O95661)(Met1-Lys225) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DIRAS3 recombinant protein

Background: ARHI, also known as DIRAS3, belongs to the small GTPase superfamily, Di-Ras family. ARHI gene is a novel tumor suppressor gene located on chromosome 1p31. Downregulation of ARHI expression has been detected in many types of cancer. ARHI is expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers. As a suppressor, ARHI is not only an important factor in the pathogenesis of gastric cancer, but also a potential factor for tumor aggravation. ARHI expression in gastric cancer can be employed to indicate favorable prognosis for the disease.

References

Pei XH. et al., 2011, Cell Biol Int. 35 (10): 1019-24.Lin D. et al., 2011, J Int Med Res. 39 (5): 1870-5.Wang W. et al., 2012, Oncol Rep. 27 (6): 1953-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4757772
• NCBI GeneID: 9077
• NCBI Accession #: NP_004666
• NCBI GenBank Nucleotide #: NM_004675.2
• UniProt Accession #: O95661; B3KMP3
• Molecular Weight: Predicted: 25 kDa; ; Observed: 24 kDa
• NCBI Official Full Name: GTP-binding protein Di-Ras3
• NCBI Official Synonym Full Names: DIRAS family, GTP-binding RAS-like 3
• NCBI Official Symbol: DIRAS3
• NCBI Official Synonym Symbols: ARHI; NOEY2
• NCBI Protein Information: GTP-binding protein Di-Ras3; ras homolog gene family, member I; rho-related GTP-binding protein RhoI; distinct subgroup of the Ras family member 3
• UniProt Protein Name: GTP-binding protein Di-Ras3
• Protein Family: GTP-binding protein
• UniProt Gene Name: DIRAS3
• UniProt Synonym Gene Names: ARHI; NOEY2; RHOI
• UniProt Entry Name: DIRA3_HUMAN

NCBI Description

This gene is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Uniprot Description

DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Protein type: G protein, monomeric, di-Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity