Rabbit anti-Human DIRAS3 Polyclonal Antibody | anti-DIRAS3 antibody

DIRAS3 Antibody

Cat. Num. AAA153530

• Gene Names: DIRAS3; ARHI; NOEY2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: DIRAS3 antibody is affinity chromatography purified via peptide column.
• Synonyms: DIRAS3; Polyclonal Antibody; DIRAS3 Antibody; DIRAS family GTP-binding RAS-like 3; ARHI; NOEY2 ; DIRAS family; GTP-binding RAS-like 3; anti-DIRAS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DIRAS3 antibody is human specific.
• Purity/Purification: DIRAS3 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 229

• Applicable Applications for anti-DIRAS3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 mug/ml.
• Conjugate: Unconjugated
• Immunogen: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
• Buffer: DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DIRAS3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of DIRAS3 in human testis tissue lysate with DIRAS3 antibody at 1 μg/ml.)

Related Product Information for anti-DIRAS3 antibody

DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).

NCBI and Uniprot Product Information

• NCBI GI #: 4757772
• NCBI GeneID: 9077
• NCBI Accession #: NP_004666
• NCBI GenBank Nucleotide #: NM_004675.2
• UniProt Accession #: O95661; B3KMP3
• Molecular Weight: Predicted: 25 kDa; ; Observed: 24 kDa
• NCBI Official Full Name: GTP-binding protein Di-Ras3
• NCBI Official Synonym Full Names: DIRAS family, GTP-binding RAS-like 3
• NCBI Official Symbol: DIRAS3
• NCBI Official Synonym Symbols: ARHI; NOEY2
• NCBI Protein Information: GTP-binding protein Di-Ras3; ras homolog gene family, member I; rho-related GTP-binding protein RhoI; distinct subgroup of the Ras family member 3
• UniProt Protein Name: GTP-binding protein Di-Ras3
• Protein Family: GTP-binding protein
• UniProt Gene Name: DIRAS3
• UniProt Synonym Gene Names: ARHI; NOEY2; RHOI
• UniProt Entry Name: DIRA3_HUMAN

NCBI Description

This gene is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Uniprot Description

DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Protein type: G protein, monomeric, di-Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity