Rabbit Dicer1 Polyclonal Antibody | anti-DICER1 antibody

Anti-Dicer1 Antibody

Cat. Num. AAA416097

• Gene Names: DICER1; DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Dicer1; Polyclonal Antibody; Anti-Dicer1 Antibody; anti-DICER1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1922

• Applicable Applications for anti-DICER1 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA titer: 1:1,000-1:10,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Immunohistochemistry (IHC)

Related Product Information for anti-DICER1 antibody

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene.

References

(1) Tabara,H., Yigit,E., Siomi,H.,et al.The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans.Cell 109 (7), 861-871 (2002)

NCBI and Uniprot Product Information

• NCBI GI #: 29294651
• NCBI GeneID: 23405
• NCBI Accession #: NP_803187
• NCBI GenBank Nucleotide #: NM_177438.2
• Molecular Weight: 92,726 Da
• NCBI Official Full Name: endoribonuclease Dicer isoform 1
• NCBI Official Synonym Full Names: dicer 1, ribonuclease type III
• NCBI Official Symbol: DICER1
• NCBI Official Synonym Symbols: DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
• NCBI Protein Information: endoribonuclease Dicer; Dicer1, Dcr-1 homolog; dicer 1, double-stranded RNA-specific endoribonuclease; helicase MOI; helicase with RNAse motif
• UniProt Protein Name: Endoribonuclease Dicer
• Protein Family: Endoribonuclease
• UniProt Gene Name: DICER1
• UniProt Entry Name: DICER_HUMAN

NCBI Description

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: RNA processing; RNA-binding; EC 3.1.26.3; Cell development/differentiation; Helicase; Ribonuclease

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: growth cone; axon; dendrite; cytoplasm; ER-Golgi intermediate compartment; nucleus; cytosol

Molecular Function: endoribonuclease activity; protein binding; ribonuclease III activity; miRNA binding; metal ion binding; double-stranded RNA binding; deoxyribonuclease I activity; helicase activity; ATP binding

Biological Process: zygote asymmetric cell division; regulation of neuron differentiation; regulation of cell cycle; regulation of oligodendrocyte differentiation; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of transcription from RNA polymerase II promoter; olfactory bulb interneuron differentiation; embryonic hindlimb morphogenesis; post-embryonic development; cardiac muscle cell development; pre-microRNA processing; angiogenesis; DNA fragmentation during apoptosis; defense response to virus; regulation of viral genome replication; spleen development; positive regulation of myelination; positive regulation of Schwann cell differentiation; hair follicle morphogenesis; inner ear receptor cell development; multicellular organism growth; RNA interference, targeting of mRNA for destruction; mRNA stabilization; stem cell maintenance; RNA interference, conversion of ds siRNA to ss siRNA; spindle assembly; spinal cord motor neuron differentiation; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; branching morphogenesis of a tube; cartilage development; gene expression; nerve development; cerebral cortex development; RNA interference, production of siRNA; reproductive structure development; neurite morphogenesis; lung development; myoblast cell differentiation involved in skeletal muscle regeneration

Disease: Pleuropulmonary Blastoma; Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Rhabdomyosarcoma, Embryonal, 2