Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin heavy chain isoform 5A (KIF5A) Standard Curve.)
Human Kinesin Heavy Chain Isoform 5A (KIF5A) ELISA Kit | KIF5A elisa kit
Human Kinesin Heavy Chain Isoform 5A (KIF5A) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin heavy chain isoform 5A (KIF5A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinesin heavy chain isoform 5A (KIF5A) Standard Curve.)
Background: The predicted 1,032-amino acid protein has the characteristic features of a KHC, as well as a unique C-terminal stretch of 69 amino acids. The amino acid sequence of NKHC is 65% and 54% identical to the amino acid sequences of KNS1 and the Drosophila KHC. Northern blot analysis of rat tissues detected Nkhc expression only in brain, where multiple transcripts were found. Immunoblot analysis of rat tissue extracts using antibodies specific for NKHC detected a doublet of approximately 120 and 133 kD only in brain and sciatic nerve tissue. By indirect Immunofluorescence, the authors showed that NKHC is distributed throughout the central nervous system but is highly enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC is concentrated in the cell body, particularly in the perinuclear region.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]
Uniprot Description
KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.
Protein type: Motor; Microtubule-binding
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: kinesin complex; microtubule; membrane; perinuclear region of cytoplasm; cytosol
Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding
Biological Process: axon guidance; synaptic transmission; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement
Disease: Spastic Paraplegia 10, Autosomal Dominant
Research Articles on KIF5A
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Product Notes
The Human KIF5A kif5a (Catalog #AAA9715580) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715580 ELISA Kit recognizes Human KIF5A. It is sometimes possible for the material contained within the vial of "Kinesin Heavy Chain Isoform 5A (KIF5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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