Clusterin (CLU) Recombinant Protein | CLU recombinant protein

Recombinant Clusterin (CLU)

Cat. Num. AAA2009043

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Clusterin (CLU); Recombinant Clusterin (CLU); CLU recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-IM PFPLFTDLNY HDMFQPFFDM IHQAQQAMDA HLHRIPYHFP EAGVPENSND RAVCKEIRHN STGCLRMKDQ CEKCREILSV DCSASNSSQM QLRQELYTSL QMAEKFSKLY DQLLQSYQQK MLNTSSLLKQ LNEQFSWVSQ LANLTQNDDR YYLQVTTVNS HGSDPSVPSG LTKVVVKLFD SYPITLIIPQ EVSDPKFMET VAEEALQQYR QRSREE
• Sequence Length: 446

• Applicable Applications for CLU recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Ile229~Glu446 (Accession # Q29549) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47522770
• NCBI GeneID: 397025
• NCBI Accession #: NP_999136.1
• NCBI GenBank Nucleotide #: NM_213971.1
• UniProt Accession #: Q29549
• Molecular Weight: 26.6kDa
• NCBI Official Full Name: clusterin
• NCBI Official Symbol: CLU
• NCBI Protein Information: clusterin; CLI; CP40; complement cytolysis inhibitor
• UniProt Protein Name: Clusterin
• Protein Family: Clusterin
• UniProt Gene Name: CLU
• UniProt Synonym Gene Names: CLI
• UniProt Entry Name: CLUS_PIG

NCBI Description

This gene encodes a protein that is similar to the human clusterin protein, a secreted chaperone that can also be found in the cell cytosol under certain stress conditions. The human protein has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. [provided by RefSeq, May 2011]

Uniprot Description

Function: Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation

By similarity.

Subunit structure: Antiparallel disulfide-linked heterodimer of an alpha chain and a beta chain. Self-associates and forms higher oligomers. Interacts with a broad range of misfolded proteins, including APP, APOC2 and LYZ. Slightly acidic pH promotes interaction with misfolded proteins. Forms high-molecular weight oligomers upon interaction with misfolded proteins. Interacts with APOA1, LRP2, CLUAP1 AND PON1. Interacts with the complement complex. Interacts with SYVN1, COMMD1, BTRC, CUL1 and with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes. Interacts (via alpha chain) with BAX in stressed cells, where BAX undergoes a conformation change leading to association with the mitochondrial membrane. Does not interact with BAX in unstressed cells. Interacts (via alpha chain) with XRCC6

By similarity. Found in a complex with LTF, CLU, EPPIN and SEMG1

By similarity.

Subcellular location: Secreted. Cytoplasmic vesicle › secretory vesicle › chromaffin granule. Nucleus

By similarity. Cytoplasm

By similarity. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cytoplasm › cytosol

By similarity. Microsome

By similarity. Endoplasmic reticulum

By similarity. Note: Present in chromaffin granules. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis

By similarity.

Tissue specificity: Highest levels in brain and liver; lower levels are detected in other tissues, including the aorta. Abundant in senescent porcine pituitary colloids. Ref.2

Post-translational modification: Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate

By similarity.Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen

By similarity.Polyubiquitinated, leading to proteasomal degradation

By similarity.

Sequence similarities: Belongs to the clusterin family.