Typical Testing Data/Standard Curve (for reference only)
Mouse Collectrin (TMEM27) ELISA Kit | TMEM27 elisa kit
Mouse Collectrin (TMEM27) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TMEM27 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-TMEM27 antibody and an TMEM27-HRP conjugate. The assay sample and buffer are incubated together with TMEM27-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMEM27 concentration since TMEM27 from samples and TMEM27-HRP conjugate compete for the anti-TMEM27 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMEM27 from the sample, fewer sites are left to bind TMEM27-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMEM27 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]
Uniprot Description
TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: brush border membrane; cytoplasm; integral to membrane
Molecular Function: peptidyl-dipeptidase activity; protein binding; metallopeptidase activity
Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport
Research Articles on TMEM27
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Product Notes
The Mouse TMEM27 tmem27 (Catalog #AAA7233927) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233927 ELISA Kit recognizes Mouse TMEM27. It is sometimes possible for the material contained within the vial of "Collectrin (TMEM27), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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