Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 2 (DPP7) Standard Curve.)
Human Dipeptidyl Peptidase 2 (DPP7) ELISA Kit | DPP7 elisa kit
Human Dipeptidyl Peptidase 2 (DPP7) ELISA Kit
Gene Names
DPP7; QPP; DPP2; DPPII
Reactivity
Human
Synonyms
Dipeptidyl Peptidase 2 (DPP7); Human Dipeptidyl Peptidase 2 (DPP7) ELISA Kit; DPP7; DPP2; DPPII; QPP; carboxytripeptidase; dipeptidyl aminopeptidase; dipeptidyl arylamidase; dipeptidyl peptidase 7; dipeptidyl-peptidase; quiescent cell proline dipeptidase; DPP7 elisa kit
Reactivity
Human
Specificity
Human Dipeptidyl peptidase 2 (DPP7) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPP7. No significant cross-reactivity or interference between Human DPP7 and analogues was observed.
Sequence Length
492
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 2 (DPP7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 2 (DPP7) Standard Curve.)
Related Product Information for DPP7 elisa kit
Description: This Human Dipeptidyl peptidase 2 (DPP7) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP7 in samples. An antibody specific for DPP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dipeptidyl-peptidase 2 is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.predicted 492-amino acid protein has a calculated molecular mass of 54.3 kD and contains the consensus sequence for the active-site serine residue of serine-type proteases. It shares 42% amino acid identity with the carboxypeptidase PRCP, with higher identity at the putative active-site residues. Northern blot analysis detected a 1.7-kb transcript in PBMCs and Jurkat T cells. SDS-PAGE analysis showed expression of a 58-kD protein.
Background: Dipeptidyl-peptidase 2 is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.predicted 492-amino acid protein has a calculated molecular mass of 54.3 kD and contains the consensus sequence for the active-site serine residue of serine-type proteases. It shares 42% amino acid identity with the carboxypeptidase PRCP, with higher identity at the putative active-site residues. Northern blot analysis detected a 1.7-kb transcript in PBMCs and Jurkat T cells. SDS-PAGE analysis showed expression of a 58-kD protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dipeptidyl peptidase 2 preproprotein
NCBI Official Synonym Full Names
dipeptidyl peptidase 7
NCBI Official Symbol
DPP7
NCBI Official Synonym Symbols
QPP; DPP2; DPPII
NCBI Protein Information
dipeptidyl peptidase 2
UniProt Protein Name
Dipeptidyl peptidase 2
Protein Family
UniProt Gene Name
DPP7
UniProt Synonym Gene Names
DPP2; QPP
UniProt Entry Name
DPP2_HUMAN
NCBI Description
The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH. [provided by RefSeq, Jul 2008]
Research Articles on DPP7
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Product Notes
The Human DPP7 dpp7 (Catalog #AAA9715647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715647 ELISA Kit recognizes Human DPP7. It is sometimes possible for the material contained within the vial of "Dipeptidyl Peptidase 2 (DPP7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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