Rabbit anti-Human, Mouse DGKK Polyclonal Antibody | anti-DGKK antibody

DGKK, CT (DGKK, Diacylglycerol kinase kappa, 142kD diacylglycerol kinase, Diglyceride kinase kappa) (Biotin)

Cat. Num. AAA6291895

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DGKK; Polyclonal Antibody; CT (DGKK; Diacylglycerol kinase kappa; 142kD diacylglycerol kinase; Diglyceride kinase kappa) (Biotin); Diglyceride kinase kappa; anti-DGKK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DGKK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DGKK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1241-1271 amino acids from the C-terminal region of human DGKK.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DGKK Antibody (C-term) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DGKK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DGKK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DGKK antibody

DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).

Product Categories/Family for anti-DGKK antibody

Antibodies; Protein Kinases

References

Imai, S., et al. J. Biol. Chem. 280(48):39870-39881(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 62000702
• NCBI GeneID: 139189
• NCBI Accession #: NP_001013764.1
• NCBI GenBank Nucleotide #: NM_001013742.2
• UniProt Accession #: Q5KSL6; B2RP91
• Molecular Weight: 141,829 Da
• NCBI Official Full Name: diacylglycerol kinase kappa
• NCBI Official Synonym Full Names: diacylglycerol kinase, kappa
• NCBI Official Symbol: DGKK
• NCBI Protein Information: diacylglycerol kinase kappa; DGK-kappa; DAG kinase kappa; diglyceride kinase kappa; 142 kDa diacylglycerol kinase
• UniProt Protein Name: Diacylglycerol kinase kappa
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKK
• UniProt Synonym Gene Names: DAG kinase kappa; DGK-kappa
• UniProt Entry Name: DGKK_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011]

Uniprot Description

DGKK: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: cytoplasm; plasma membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; diacylglycerol metabolic process; response to oxidative stress; blood coagulation; phosphorylation