RYBP recombinant protein

RYBP, 1-228aa, Human, His tag, E Coli

Cat. Num. AAA204274

• Gene Names: RYBP; AAP1; DEDAF; YEAF1; APAP-1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RYBP; 1-228aa; Human; His tag; E Coli; RING1 and YY1 binding protein; AAP1; DEDAF; YEAF1; RYBP recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 20% glycerol, 1mM DTT, 100mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF
• Sequence Length: 228

• Applicable Applications for RYBP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RYBP recombinant protein

RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG). This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RYBP recombinant protein

Apoptosis

References

Chen D, et al. (2008) EMBO Rep. 10 (2):166-72. ; Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952

NCBI and Uniprot Product Information

• NCBI GI #: 112363092
• NCBI GeneID: 23429
• NCBI Accession #: NP_036366
• NCBI GenBank Nucleotide #: NM_012234.6
• UniProt Accession #: Q8N488; Q9P2W5; Q9UMW4
• Molecular Weight: 27.4kDa (252aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: RYBP
• NCBI Official Synonym Symbols: AAP1; DEDAF; YEAF1; APAP-1
• NCBI Protein Information: RING1 and YY1-binding protein
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RYBP
• UniProt Synonym Gene Names: DEDAF; YEAF1; APAP-1; DED-associated factor

Uniprot Description

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA ().