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Western Blot (WB) (Western blot analysis of DGKK Antibody (C-term) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse DGKK Polyclonal Antibody | anti-DGKK antibody

DGKK Antibody (C-term)

Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DGKK; Polyclonal Antibody; DGKK Antibody (C-term); Diacylglycerol kinase kappa; DAG kinase kappa; 142 kDa diacylglycerol kinase; Diglyceride kinase kappa; DGK-kappa; anti-DGKK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DGKK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1232-1261 amino acids from the C-terminal region of human DGKK.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
1232-1261
Sequence Length
1271
Applicable Applications for anti-DGKK antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
Function
Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA)
Cellular Location
Cytoplasm. Membrane; Peripheral membrane protein
Tissue Location
Expressed in testis, and to a lesser extent in placenta.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of DGKK Antibody (C-term) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of DGKK Antibody (C-term) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DGKK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DGKK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (DGKK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DGKK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-DGKK antibody
DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).
Product Categories/Family for anti-DGKK antibody
References
Imai, S., et al. J. Biol. Chem. 280(48):39870-39881(2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
141829
NCBI Official Full Name
diacylglycerol kinase kappa
NCBI Official Synonym Full Names
diacylglycerol kinase, kappa
NCBI Official Symbol
DGKK
NCBI Protein Information
diacylglycerol kinase kappa
UniProt Protein Name
Diacylglycerol kinase kappa
Protein Family
UniProt Gene Name
DGKK
UniProt Synonym Gene Names
DAG kinase kappa; DGK-kappa
UniProt Entry Name
DGKK_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011]

Uniprot Description

DGKK: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: cytoplasm; plasma membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; diacylglycerol metabolic process; response to oxidative stress; blood coagulation; phosphorylation

Research Articles on DGKK

Similar Products

Product Notes

The DGKK dgkk (Catalog #AAA9206606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1232-1261. The DGKK Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGKK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the DGKK dgkk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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