Western Blot (WB)
(Western blotAll lanes: DFFA antibody at 10ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 37,30 kDaObserved band size: 37 kDa)
Rabbit anti-Human DFFA Polyclonal Antibody | anti-DFFA antibody
DFFA Antibody
IHC:1:20-1:200
Western Blot (WB)
(Western blotAll lanes: DFFA antibody at 10ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 37,30 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western blotAll lanes: DFFA antibody at 10ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 37,30 kDaObserved band size: 37 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1494539 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1494539 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1494539 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1494539 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating DFFA in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1494539 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1494539(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating DFFA in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1494539 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1494539(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase
Chromosomal Location of Human Ortholog: 1p36.3-p36.2
Cellular Component: cytoplasm; cytosol; lipid particle; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: apoptosis; cell structure disassembly during apoptosis; DNA fragmentation during apoptosis; positive regulation of apoptosis; programmed cell death
Research Articles on DFFA
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Product Notes
The DFFA dffa (Catalog #AAA1494539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DFFA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DFFA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: WB:1:500-5000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the DFFA dffa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DFFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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