Mouse anti-Human BAG4 Monoclonal Antibody | anti-BAG4 antibody

BAG4 (SODD, BAG Family Molecular Chaperone Regulator 4, Bcl-2-associated Athanogene 4, Silencer of Death Domains) (Biotin)

Cat. Num. AAA6140780

• Gene Names: BAG4; SODD; BAG-4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAG4; Monoclonal Antibody; BAG4 (SODD; BAG Family Molecular Chaperone Regulator 4; Bcl-2-associated Athanogene 4; Silencer of Death Domains) (Biotin); anti-BAG4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2G9
• Specificity: Recognizes human BAG4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 4297

• Applicable Applications for anti-BAG4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant protein corresponding to aa1-457 from BAG4 (AAH38505) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPISWRVRGGGPAETTWLGEGGGGDGYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKELLELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (76.01kD).)

Western Blot (WB)

(Western Blot analysis of BAG4 expression in transfected 293T cell line by BAG4 monoclonal antibody Lane 1: BAG4 transfected lysate (49.6kD). Lane 2: Non-transfected lysate. )

Testing Data

(Detection limit for recombinant GST tagged BAG4 is ~3ng/ml as a capture antibody.)

Related Product Information for anti-BAG4 antibody

BAG4 is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost.

Product Categories/Family for anti-BAG4 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 23620498
• NCBI GeneID: 9530
• NCBI Official Full Name: Homo sapiens BCL2-associated athanogene 4, mRNA
• NCBI Official Synonym Full Names: BAG cochaperone 4
• NCBI Official Symbol: BAG4
• NCBI Official Synonym Symbols: SODD; BAG-4
• NCBI Protein Information: BAG family molecular chaperone regulator 4
• Protein Family: BAG family molecular chaperone regulator

NCBI Description

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]