Western Blot (WB)
(Detection of Human DEC1 by Western Blot. Samples: Whole cell lysate from HeLa (50ug for WB) cells. Antibodies: MBS615769 used for WB at 0.1ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes.)
Rabbit anti-Human DEC1 Polyclonal Antibody | anti-DEC1 antibody
DEC1 (DEC-1, Differentially Expressed in Chondrocytes, Differentiated Embryo Chrondrocyte Expressed Gene 1, BHLHB2, Basic Helix-Loop-Helix Domain Containing Class B 2, SHARP2, SHARP-2, STRA13, STRA14)
Gene Names
DEC1; CTS9
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
DEC1; Polyclonal Antibody; DEC1 (DEC-1; Differentially Expressed in Chondrocytes; Differentiated Embryo Chrondrocyte Expressed Gene 1; BHLHB2; Basic Helix-Loop-Helix Domain Containing Class B 2; SHARP2; SHARP-2; STRA13; STRA14); Anti -DEC1 (DEC-1; anti-DEC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DEC1.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Applicable Applications for anti-DEC1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:5000-1:15,000
Immunoprecipitation: 1-4ug/mg of lysate
Dilution: Western Blot: 1:5000-1:15,000
Immunoprecipitation: 1-4ug/mg of lysate
Immunogen
Synthetic peptide corresponding to a region between aa350-412 of the C-terminus of human DEC1 (Accession # NP_003661.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Detection of Human DEC1 by Western Blot. Samples: Whole cell lysate from HeLa (50ug for WB) cells. Antibodies: MBS615769 used for WB at 0.1ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes.)
Western Blot (WB)
(Detection of Human DEC1 by Western Blot. Samples: Whole cell lysate from HeLa (50ug for WB) cells. Antibodies: MBS615769 used for WB at 0.1ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes.)
Western Blot (WB)
(Detection of Human DEC1 by WB and IP. Samples: Whole cell lysate from HeLa (50ug for WB) and 293T (1mg/IP, 20% of IP loaded) cells. MBS615769 used for WB at 1ug/ml and for IP at 3ug/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds.)
Western Blot (WB)
(Detection of Human DEC1 by WB and IP. Samples: Whole cell lysate from HeLa (50ug for WB) and 293T (1mg/IP, 20% of IP loaded) cells. MBS615769 used for WB at 1ug/ml and for IP at 3ug/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds.)
Related Product Information for anti-DEC1 antibody
Differentiated embryo-chondrocyte expressed gene 1 (DEC1) is an important protein involved in embryonic cell differentiation, proliferation, and apoptosis. It has also recently been shown to be regulated by hypoxia. The loss of DEC1 expression may be an early event in the development of lung cancer, while DEC1 may be up-regulated by hypoxia in other cancers and in more extreme hypoxia it may have a role in cell death.
Product Categories/Family for anti-DEC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,542 Da
NCBI Official Full Name
deleted in esophageal cancer 1
NCBI Official Synonym Full Names
deleted in esophageal cancer 1
NCBI Official Symbol
DEC1
NCBI Official Synonym Symbols
CTS9
NCBI Protein Information
deleted in esophageal cancer 1; OTTHUMP00000021982; candidate tumor suppressor CTS9
UniProt Protein Name
Deleted in esophageal cancer 1
UniProt Gene Name
DEC1
UniProt Synonym Gene Names
CTS9
UniProt Entry Name
DEC1_HUMAN
NCBI Description
The function of this gene is not known. This gene is located in a region commonly deleted in esophageal squamous cell carcinomas. Gene expression is reduced or absent in these carcinomas and thus this is a candidate tumor suppressor gene for esophageal squamous cell carcinomas. [provided by RefSeq]
Uniprot Description
DEC1: Candidate tumor supressor.
Protein type: Tumor suppressor
Chromosomal Location of Human Ortholog: 9q32
Biological Process: negative regulation of cell proliferation
Disease: Esophageal Cancer
Research Articles on DEC1
Similar Products
Product Notes
The DEC1 dec1 (Catalog #AAA615769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEC1 (DEC-1, Differentially Expressed in Chondrocytes, Differentiated Embryo Chrondrocyte Expressed Gene 1, BHLHB2, Basic Helix-Loop-Helix Domain Containing Class B 2, SHARP2, SHARP-2, STRA13, STRA14) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DEC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:5000-1:15,000 Immunoprecipitation: 1-4ug/mg of lysate. Researchers should empirically determine the suitability of the DEC1 dec1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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