Mouse anti-Human HAX1 Monoclonal Antibody | anti-HAX1 antibody

HAX1 antibody

Cat. Num. AAA200151

• Gene Names: HAX1; SCN3; HS1BP1; HCLSBP1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: By protein-G affinity chromatography
• Synonyms: HAX1; Monoclonal Antibody; HAX1 antibody; HCLS1 associated protein X-1; HS1BP1; HCLSBP1; HCLS1 (and PKD2) associated protein; anti-HAX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: AT3C5
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 279

• Applicable Applications for anti-HAX1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000 ~ 2000.; Recommended starting dilution is 1:1000.
• Antigen Species: Human
• Immunogen: Recombinant human HAX1 (1-279aa) purified from E. coli
• Clone Note: Anti-human HAX1 mAb, clone AT3C5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HAX1 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The HeLa cell lysate (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Raji cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of HAX1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HAX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-HAX1 antibody

HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the polycystic kidney disease 2 (PKD2) gene and with the F-actin-binding protein, cortactin. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.

Product Categories/Family for anti-HAX1 antibody

Metabolism

References

Klein C, et al., (2007) Nat Genet. 39 (1):86-92; Yin H, et al., (2001) Cytokine. 15 (3):122-37

NCBI and Uniprot Product Information

• NCBI GI #: 13435356
• NCBI GeneID: 10456
• NCBI Accession #: NP_006109
• NCBI GenBank Nucleotide #: NM_006118.3
• UniProt Accession #: O00165; Q5VYD5; Q5VYD7; Q96AU4; Q9BS80; A8W4W9; A8W4X0; B4DUJ7
• Molecular Weight: 21,801 Da
• NCBI Official Full Name: HCLS1-associated protein X-1 isoform a
• NCBI Official Synonym Full Names: HCLS1 associated protein X-1
• NCBI Official Symbol: HAX1
• NCBI Official Synonym Symbols: SCN3; HS1BP1; HCLSBP1
• NCBI Protein Information: HCLS1-associated protein X-1
• UniProt Protein Name: HCLS1-associated protein X-1
• Protein Family: HCLS1-associated protein
• UniProt Gene Name: HAX1
• UniProt Synonym Gene Names: HS1BP1; HAX-1; HSP1BP-1

NCBI Description

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed:26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed:26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.