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Testing Data (Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC)

Mouse CD63 Monoclonal Antibody | anti-CD63 antibody

MOUSE ANTI RAT CD63:FITC

Applications
Flow Cytometry, Functional Assay
Synonyms
CD63; Monoclonal Antibody; MOUSE ANTI RAT CD63:FITC; anti-CD63 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AD1
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-CD63 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC)

Testing Data (Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC)

Testing Data

(Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor488 (MBS219505A488))

Testing Data (Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor488 (MBS219505A488))

Testing Data

(Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor647 (MBS219505A647))

Testing Data (Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor647 (MBS219505A647))
Related Product Information for anti-CD63 antibody
Mouse anti Rat CD63 antibody, clone AD1 specifically recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is translocated to the cell surface upon activation of platelets, and therefore a potential indicator of platelet activation. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1. Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release (Kitani et al. 1991).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,699 Da
NCBI Official Full Name
CD63 antigen
NCBI Official Synonym Full Names
Cd63 molecule
NCBI Official Symbol
Cd63
NCBI Protein Information
CD63 antigen; mast cell antigen AD1
UniProt Protein Name
CD63 antigen
Protein Family
UniProt Gene Name
Cd63
UniProt Entry Name
CD63_RAT

NCBI Description

human homolog facilitates endocytosis of H,K-ATPase beta-subunit; may play a role in protein trafficking [RGD, Feb 2006]

Uniprot Description

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli ().

Research Articles on CD63

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Product Notes

The CD63 cd63 (Catalog #AAA219809) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD63 cd63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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