DDX56 recombinant protein

DDX56, 1-547aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205310

• Gene Names: DDX56; DDX21; DDX26; NOH61
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: DDX56; 1-547aa; Human; His tag; E Coli (Denatured); probable ATP-dependent RNA helicase DDX56 isoform 1; DDX21; DDX26; NOH61; DDX56 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEDSEAL GFEHMGLDPR LLQAVTDLGW SRPTLIQEKA IPLALEGKDL LARARTGSGK TAAYAIPMLQ LLLHRKATGP VVEQAVRGLV LVPTKELARQ AQSMIQQLAT YCARDVRVAN VSAAEDSVSQ RAVLMEKPDV VVGTPSRILS HLQQDSLKLR DSLELLVVDE ADLLFSFGFE EELKSLLCHL PRIYQAFLMS ATFNEDVQAL KELILHNPVT LKLQESQLPG PDQLQQFQVV CETEEDKFLL LYALLKLSLI RGKSLLFVNT LERSYRLRLF LEQFSIPTCV LNGELPLRSR CHIISQFNQG FYDCVIATDA EVLGAPVKGK RRGRGPKGDK ASDPEAGVAR GIDFHHVSAV LNFDLPPTPE AYIHRAGRTA RANNPGIVLT FVLPTEQFHL GKIEELLSGE NRGPILLPYQ FRMEEIEGFR YRCRDAMRSV TKQAIREARL KEIKEELLHS EKLKTYFEDN PRDLQLLRHD LPLHPAVVKP HLGHVPDYLV PPALRGLVRP HKKRKKLSSS CRKAKRAKSQ NPLRSFKHKG KKFRPTAKPS
• Sequence Length: 547

• Applicable Applications for DDX56 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DDX56 recombinant protein

DDX56 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX56 shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. Recombinant human DDX56 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for DDX56 recombinant protein

Epigenomics

References

Matsuoka S., et al (2007). Science 316:1160-1166

NCBI and Uniprot Product Information

• NCBI GI #: 9506931
• NCBI GeneID: 54606
• NCBI Accession #: NP_061955
• NCBI GenBank Nucleotide #: NM_019082.3
• UniProt Accession #: Q9NY93; Q6IAE2; Q9H9I8; A4D2K9; C9JV95
• Molecular Weight: 64.0 kDa (570aa)
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX56 isoform 1
• NCBI Official Synonym Full Names: DEAD-box helicase 56
• NCBI Official Symbol: DDX56
• NCBI Official Synonym Symbols: DDX21; DDX26; NOH61
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX56
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX56
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX56
• UniProt Synonym Gene Names: DDX21; NOH61

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.