Rabbit anti-Human DDX17 Polyclonal Antibody | anti-DDX17 antibody

DDX17, NT (DDX17, Probable ATP-dependent RNA helicase DDX17, DEAD box protein 17, DEAD box protein p72, RNA-dependent helicase p72)

Cat. Num. AAA644322

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DDX17; Polyclonal Antibody; NT (DDX17; Probable ATP-dependent RNA helicase DDX17; DEAD box protein 17; DEAD box protein p72; RNA-dependent helicase p72); Anti -DDX17; anti-DDX17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DDX17 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: DDX17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55~85 amino acids from the N-terminal region of human DDX17.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX17 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). DDX17 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DDX17 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DDX17 Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDX17 antibody

DDX17 box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family.

Product Categories/Family for anti-DDX17 antibody

Antibodies; Abs to DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47678395
• UniProt Accession #: Q92841; Q69YT1; Q6ICD6; B1AHM0
• Molecular Weight: 80,272 Da
• NCBI Official Full Name: DDX17
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX17
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX17
• UniProt Entry Name: DDX17_HUMAN

Uniprot Description

DDX17: RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation. Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation. Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Transcription, coactivator/corepressor; Nucleolus; Spliceosome; RNA processing; EC 3.6.4.13; Helicase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA-dependent ATPase activity; RNA binding; transcription coactivator activity; estrogen receptor binding; RNA helicase activity; ATP binding

Biological Process: RNA processing; transcription, DNA-dependent; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter