Rabbit anti-Human DDX17 Polyclonal Antibody | anti-DDX17 antibody

DDX17, NT (DDX17, Probable ATP-dependent RNA helicase DDX17, DEAD box protein 17, DEAD box protein p72, RNA-dependent helicase p72) (FITC)

Cat. Num. AAA6291357

• Gene Names: DDX17; P72; RH70
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX17; Polyclonal Antibody; NT (DDX17; Probable ATP-dependent RNA helicase DDX17; DEAD box protein 17; DEAD box protein p72; RNA-dependent helicase p72) (FITC); RNA-dependent helicase p72; anti-DDX17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DDX17 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DDX17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55~85 amino acids from the N-terminal region of human DDX17.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX17 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). DDX17 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DDX17 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DDX17 Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDX17 antibody

DDX17 box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family.

Product Categories/Family for anti-DDX17 antibody

Antibodies; DEAD Box Proteins

References

Wortham, N.C., et.al., Oncogene 28 (46), 4053-4064 (2009). Tonevitsky, E.A., et.al., Bull. Exp. Biol. Med. 147 (6), 733-736 (2009). Wong, H.Y., et.al., Biochim. Biophys. Acta 1794 (2), 193-198 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148613854
• NCBI GeneID: 10521
• NCBI Accession #: NP_006377.2
• NCBI GenBank Nucleotide #: NM_006386
• UniProt Accession #: Q92841; Q69YT1; Q6ICD6; B1AHM0
• Molecular Weight: 72,557 Da
• NCBI Official Full Name: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 17
• NCBI Official Symbol: DDX17
• NCBI Official Synonym Symbols: P72; RH70
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX17; DEAD (Asp-Glu-Ala-Asp) box polypeptide 17; DEAD box protein p72; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD); RNA-dependent helicase p72
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX17
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX17

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]

Uniprot Description

DDX17: RNA-dependent ATPase activity. Involved in transcriptional regulation. Transcriptional coactivator for estrogen receptor ESR1. Increases ESR1 AF-1 domain-mediated transactivation. Synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and probably involved in skeletal muscle differentiation. Required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation. Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase; Nuclear receptor co-regulator; Nucleolus; RNA processing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; membrane; nuclear speck; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding; RNA helicase activity; RNA-dependent ATPase activity; transcription coactivator activity

Biological Process: alternative mRNA splicing, via spliceosome; androgen receptor signaling pathway; defense response to virus; epithelial to mesenchymal transition; intracellular estrogen receptor signaling pathway; miRNA metabolic process; myoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of alternative mRNA splicing, via spliceosome; regulation of skeletal muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; RNA processing; RNA secondary structure unwinding; RNA-mediated gene silencing; rRNA processing; transcription, DNA-dependent