Western Blot (WB)
(The DCAMKL1 Antibody (C-term) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).)
Rabbit anti-Human, Mouse DCAMKL1 Polyclonal Antibody | anti-DCLK1 antibody
DCAMKL1 Antibody (C-term)
Western Blot (WB)
(The DCAMKL1 Antibody (C-term) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).)
Western Blot (WB)
(The DCAMKL1 Antibody (C-term) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).)
Immunohistochemistry (IHC)
(Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).)
Immunohistochemistry (IHC)
(Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).)
Immunofluorescence (IF)
((C): The cell positive for DCAMKL-1, stained red (indicated by the arrow), appears at the base of the crypt. (D): Intestinal section stained for Musashi-1 (green). (E): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow). The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1. (F): Costaining of DCMAKL-1 (red, indicated by the arrow) with nuclear Hoechst 33342 (blue) staining. (G): Costaining of Musashi-1 (green) with nuclear Hoechst 33342 (blue) staining. (H): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow), costained with nuclear Hoechst 33342 (blue) staining. The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1 (yellow).)
Immunofluorescence (IF)
((C): The cell positive for DCAMKL-1, stained red (indicated by the arrow), appears at the base of the crypt. (D): Intestinal section stained for Musashi-1 (green). (E): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow). The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1. (F): Costaining of DCMAKL-1 (red, indicated by the arrow) with nuclear Hoechst 33342 (blue) staining. (G): Costaining of Musashi-1 (green) with nuclear Hoechst 33342 (blue) staining. (H): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow), costained with nuclear Hoechst 33342 (blue) staining. The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1 (yellow).)
Western Blot (WB)
(All lanes :DCAMKL1 Antibody (C-term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes :DCAMKL1 Antibody (C-term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes :DCAMKL1 Antibody (C-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes :DCAMKL1 Antibody (C-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Western Blot (WB)
(All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
Uniprot Description
DCAMKL1: a serine-threonine kinase of the CAMK family. May be involved in a calcium-signaling pathway controling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. Genetic and physical interactions with Doublecortin, which causes lissencephaly. A putative marker of quiescent stem cells. Four alternatively spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; DCAMKL family
Chromosomal Location of Human Ortholog: 13q13
Cellular Component: integral to plasma membrane
Molecular Function: protein serine/threonine kinase activity; receptor signaling protein activity; ATP binding; protein kinase activity
Biological Process: nervous system development; central nervous system development; axon extension; response to virus; dendrite morphogenesis; forebrain development; neuron migration; central nervous system projection neuron axonogenesis; endosome transport; protein amino acid phosphorylation
Research Articles on DCLK1
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Product Notes
The DCLK1 dclk1 (Catalog #AAA9214058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 690-720. The DCAMKL1 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DCAMKL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:8000. Researchers should empirically determine the suitability of the DCLK1 dclk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCAMKL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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