DCAMKL1 / DCLK1 recombinant protein

Recombinant Human DCAMKL1 / DCLK1 Protein

Cat. Num. AAA2545736

• Gene Names: DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: DCAMKL1 / DCLK1; Recombinant Human DCAMKL1 / DCLK1 Protein; RP11-113P14.1; CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; DCAMKL1 / DCLK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
• Sequence Length: 422

• Application Notes: The secreted recombinant human DCLK1 consists of 707 amino acids and predicts a molecular mass of 78.5 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gly
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DCAMKL1 / DCLK1 recombinant protein

Background: DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

Description: A DNA sequence encoding the human DCLK1 ( O15075-1) (Met 1-Val 705) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 306518604
• NCBI GeneID: 9201
• NCBI Accession #: NP_001182344.1
• NCBI GenBank Nucleotide #: NM_001195415.1
• UniProt Accession #: O15075; Q5VZY8; Q5VZZ0; Q5VZZ1; B7Z3E9
• Molecular Weight: 47,681 Da
• NCBI Official Full Name: serine/threonine-protein kinase DCLK1 isoform 2
• NCBI Official Synonym Full Names: doublecortin like kinase 1
• NCBI Official Symbol: DCLK1
• NCBI Official Synonym Symbols: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• NCBI Protein Information: serine/threonine-protein kinase DCLK1
• UniProt Protein Name: Serine/threonine-protein kinase DCLK1
• UniProt Gene Name: DCLK1
• UniProt Synonym Gene Names: DCAMKL1; DCDC3A; KIAA0369

NCBI Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]

Uniprot Description

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.