Mouse anti-Human Daxx Monoclonal Antibody | anti-DAXX antibody

Daxx (BING2, DAP 6, DAP6, Death associated protein 6, EAP 1, EAP1, ETS1 associated protein 1, Fas death domain associated protein, hDaxx, MGC126245, MGC126246)

Cat. Num. AAA604893

• Gene Names: DAXX; DAP6; EAP1; BING2; MGC126245; MGC126246
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Purified; Purified
• Synonyms: Daxx; Monoclonal Antibody; Daxx (BING2; DAP 6; DAP6; Death associated protein 6; EAP 1; EAP1; ETS1 associated protein 1; Fas death domain associated protein; hDaxx; MGC126245; MGC126246); Anti -Daxx (BING2; anti-DAXX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D345
• Specificity: Recognizes human Daxx.
• Purity/Purification: Purified; Purified
• Form/Format: Liquid. 100ug of purified antibody in PBS, pH 7.4, containing 0.05% sodium azide.

• Applicable Applications for anti-DAXX antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blotting, Immunocytochemistry.; Dilution: Western Blotting: 1:100-1:1000
• Immunogen: Recombinant human Daxx (aa 558-740).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Figure: Detection of Daxx in cell lysate of Myc-epitope tagged Daxx overexpressing TF-1 cells. Lane 1 shows endogenous Daxx detected by MBS604893.)

Immunofluorescence (IF)

(Figure: Immunofluorescence microscopy of HeLa cells transfected with pCDNA3-MycDaxx and stained with anti-Daxx (green) MAb or anti-Myc epitope MAb (blue). Left: nuclear localization of Myc-Daxx. Right: Co-transfection with pCDNA3-ASK1HA, leading to translocation of Daxx from nucleus to the cytoplasm.)

Related Product Information for anti-DAXX antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx1,2. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).

Product Categories/Family for anti-DAXX antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 2253709
• NCBI GeneID: 1616
• UniProt Accession #: Q4VX54; Q5STK8; Q53F85; Q59FG7; Q5STR5; Q9UER7
• Molecular Weight: 19,115 Da
• NCBI Official Full Name: Daxx
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2; MGC126245; MGC126246
• NCBI Protein Information: death domain-associated protein 6; hDaxx; OTTHUMP00000029289; OTTHUMP00000029290; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein
• UniProt Protein Name: Death-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Entry Name: Q4VX54_HUMAN

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Nucleolus; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; enzyme binding; protein homodimerization activity; androgen receptor binding; histone binding; p53 binding; heat shock protein binding; ubiquitin protein ligase binding; receptor signaling protein activity; protein N-terminus binding; transcription factor binding; transcription corepressor activity; protein kinase activator activity; protein kinase binding

Biological Process: viral reproduction; transcription, DNA-dependent; regulation of protein ubiquitination; apoptosis; activation of JNK activity; chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; cytokinesis after mitosis; induction of apoptosis via death domain receptors; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent