Rabbit anti-Human DAXX Polyclonal Antibody | anti-DAXX antibody

Rabbit Polyclonal (IgG) to Human DAXX

Cat. Num. AAA244837

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DAXX; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human DAXX; Anti-DAXX Antibody (aa634-683) IHC-plus; BING2; CENP-C binding protein; DAP6; Death-associated protein 6; EAP1; Fas-binding protein; HDaxx; ETS1-associated protein 1; Human DAXX; anti-DAXX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAXX Antibody detects endogenous levels of total DAXX protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 740

• Applicable Applications for anti-DAXX antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (15 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Daxx (aa634-683).
• Immunogen: DAXX antibody was raised against synthetic peptide from human Daxx (aa634-683).
• Antigen Modification: aa634-683
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-DAXX antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)

Western Blot (WB)

(Western blot of extracts from COS7 cells, treated with Serum 10% 30', using Daxx antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-DAXX antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.

Product Categories/Family for anti-DAXX antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4503257
• NCBI GeneID: 1616
• NCBI Accession #: NP_001341.1
• NCBI GenBank Nucleotide #: NM_001350.4
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5H082
• Molecular Weight: 73,589 Da
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Nucleolus

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; transcription corepressor activity; protein kinase activator activity

Biological Process: viral reproduction; transcription, DNA-dependent; regulation of protein ubiquitination; apoptosis; activation of JNK activity; chromatin remodeling; nucleosome assembly; induction of apoptosis via death domain receptors; cytokinesis after mitosis; regulation of transcription, DNA-dependent; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent