Western Blot/Blotting
(Immunoprecipitation using DAP12 antibody on MHC class I (1) and NKp44 (2) positive cells.)
Rabbit anti-Human, Mouse DAP12 Polyclonal Antibody | anti-DAP12 antibody
DAP12
Gene Names
Tyrobp; Ly83; DAP12; KARAP
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Ammonium Sulfate Precipitation
Synonyms
DAP12; Polyclonal Antibody; TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein; KARAP; TYROBP; anti-DAP12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Ammonium Sulfate Precipitation
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
114
Applicable Applications for anti-DAP12 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Antibody can be used for immunoprecipitation (2-4 ug/mL protein lysate)
Optimal concentration should be evaluated by serial dilutions.
Optimal concentration should be evaluated by serial dilutions.
Source
Rabbit immunized with recombiant DAP12
Immunogen
Recombinant DAP12 protein
Positive Control
NKp44 positive cells
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Western Blot/Blotting
(Immunoprecipitation using DAP12 antibody on MHC class I (1) and NKp44 (2) positive cells.)
Western Blot/Blotting
(Immunoprecipitation using DAP12 antibody on MHC class I (1) and NKp44 (2) positive cells.)
Related Product Information for anti-DAP12 antibody
Background: DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (1,2). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (3). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (4,5), as well as presenile dementia and bone cysts in humans (6).
Product Categories/Family for anti-DAP12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
TYRO protein tyrosine kinase-binding protein
NCBI Official Synonym Full Names
TYRO protein tyrosine kinase binding protein
NCBI Official Symbol
Tyrobp
NCBI Official Synonym Symbols
Ly83; DAP12; KARAP
NCBI Protein Information
TYRO protein tyrosine kinase-binding protein; KAR-associated protein; DNAX-activation protein 12; killer-activating receptor-associated protein; killer cell activating receptor associated protein
UniProt Protein Name
TYRO protein tyrosine kinase-binding protein
UniProt Gene Name
Tyrobp
UniProt Synonym Gene Names
Dap12; Karap; KAR-associated protein
UniProt Entry Name
TYOBP_MOUSE
Uniprot Description
Function: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Ref.7
Subunit structure: Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with SIGLEC14
By similarity. Interacts with CLECSF5. Interacts with CD300LB and CD300D. Interacts with CD300E. Interacts with KLRC2 and KIR2DS3
By similarity. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophils and macrophages integrin-mediated activation. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10
Subcellular location: Membrane; Single-pass type I membrane protein.
Post-translational modification: Tyrosine phosphorylated.
Sequence similarities: Belongs to the TYROBP family.Contains 1 ITAM domain.
Research Articles on DAP12
1. the Siglec-15-DAP12-Syk-signaling cascade plays a critical role in functional osteoclast formation.
Similar Products
Product Notes
The DAP12 tyrobp (Catalog #AAA395757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAP12 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DAP12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Antibody can be used for immunoprecipitation (2-4 ug/mL protein lysate)Optimal concentration should be evaluated by serial dilutions. Researchers should empirically determine the suitability of the DAP12 tyrobp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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