Rabbit anti-Human DAP12 Polyclonal Antibody | anti-DAP12 antibody

DAP12 Polyclonal Antibody

Cat. Num. AAA3012573

• Gene Names: TYROBP; DAP12; KARAP; PLOSL; PLOSL1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: DAP12; Polyclonal Antibody; DAP12 Polyclonal Antibody; KAR-associated protein; TYROBP; KARAP; DAP 12; DNAX activation protein 12; DNAX-activation protein 12; Killer activating receptor associated protein; Killer-activating receptor-associated protein; PLOSL; TYOBP; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; anti-DAP12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAP12 polyclonal antibody detects endogenous levels of DAP12 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 113

• Applicable Applications for anti-DAP12 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within human DAP12 aa 60-100.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-DAP12 antibody

Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 4507755
• NCBI GeneID: 7305
• NCBI Accession #: NP_003323.1
• NCBI GenBank Nucleotide #: NP_003323.1
• UniProt Accession #: O43914
• Molecular Weight: 12kDa
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein isoform 1
• NCBI Official Synonym Full Names: transmembrane immune signaling adaptor TYROBP
• NCBI Official Symbol: TYROBP
• NCBI Official Synonym Symbols: DAP12; KARAP; PLOSL; PLOSL1
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: TYROBP
• UniProt Synonym Gene Names: DAP12; KARAP; KAR-associated protein
• UniProt Entry Name: TYOBP_HUMAN

NCBI Description

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; receptor signaling protein activity; receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; regulation of immune response; neutrophil activation during immune response; macrophage activation during immune response; innate immune response; cellular defense response; signal transduction

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy