Rabbit anti-Human DAP12 Polyclonal Antibody | anti-DAP12 antibody

DAP12 Antibody

Cat. Num. AAA840274

• Gene Names: Tyrobp; Ly83; DAP12; KARAP
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: DAP12; Polyclonal Antibody; DAP12 Antibody; DAP-12; DAP 12; TYRO protein tyrosine kinase binding protein; TYROBP; anti-DAP12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-DAP12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 114

• Applicable Applications for anti-DAP12 antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 94 of mouse DAP-12
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-DAP12 antibody

Background: DAP12 (KARAP) is a natural killer (NK) cell-expressed cell surface receptor and belongs to the immunoglobulin and C-type lectin superfamily. Unlike other members of this family which possess immunoreceptor tyrosine-based inhibitory motifs (ITIM), DAP12 contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and is present as a disulphide-bonded homodimer. Crosslinking of DAP 12-KIR complexes (membrane glycoproteins of the killer-cell inhibitory receptor family without an ITIM in their cytoplasmic domain) results in cellular activation.

NCBI and Uniprot Product Information

• NCBI GI #: 6755915
• NCBI GeneID: 22177
• NCBI Accession #: NP_035792.1
• NCBI GenBank Nucleotide #: NM_011662.2
• UniProt Accession #: O54885; O88603
• Molecular Weight: 12,367 Da
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein
• NCBI Official Synonym Full Names: TYRO protein tyrosine kinase binding protein
• NCBI Official Symbol: Tyrobp
• NCBI Official Synonym Symbols: Ly83; DAP12; KARAP
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: Tyrobp
• UniProt Synonym Gene Names: Dap12; Karap; KAR-associated protein
• UniProt Entry Name: TYOBP_MOUSE

Uniprot Description

TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: cell surface; membrane; integral to membrane

Molecular Function: identical protein binding; protein binding; receptor binding

Biological Process: integrin-mediated signaling pathway; neutrophil activation during immune response; macrophage activation during immune response