Human TYRO protein tyrosine kinase-binding protein ELISA Kit | TYROBP elisa kit

Human TYRO protein tyrosine kinase-binding protein ELISA Kit

Cat. Num. AAA764281

• Gene Names: TYROBP; DAP12; KARAP; PLOSL
• Reactivity: Human
• Synonyms: TYRO protein tyrosine kinase-binding protein; Human TYRO protein tyrosine kinase-binding protein ELISA Kit; TYROBP (TYRO protein tyrosine kinase-binding protein)/DAP12/KARAP/PLOSL/DNAX-activation protein 12/PLO-SL/KAR-associated protein/killer activating receptor associated protein/Killer-activating receptor-associated protein/TYRO protein tyrosine kinase binding protein; TYROBP elisa kit

• Reactivity: Human
• Sequence Length: 113

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TYROBP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TYROBPantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TYROBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TYROBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman TYROBPcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507755
• NCBI GeneID: 7305
• NCBI Accession #: NP_003323.1
• NCBI GenBank Nucleotide #: NM_003332.3
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein isoform 1
• NCBI Official Synonym Full Names: TYRO protein tyrosine kinase binding protein
• NCBI Official Symbol: TYROBP
• NCBI Official Synonym Symbols: DAP12; KARAP; PLOSL
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• Protein Family: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: TYROBP
• UniProt Synonym Gene Names: DAP12; KARAP; KAR-associated protein
• UniProt Entry Name: TYOBP_HUMAN

NCBI Description

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell surface; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: identical protein binding; protein binding; receptor binding; receptor signaling protein activity

Biological Process: axon guidance; cellular defense response; innate immune response; integrin-mediated signaling pathway; macrophage activation during immune response; neutrophil activation during immune response; regulation of immune response; signal transduction

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy