Rabbit anti-Human DACH2 Polyclonal Antibody | anti-DACH2 antibody

DACH2, NT (DACH2, Dachshund homolog 2) (FITC)

Cat. Num. AAA6290697

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DACH2; Polyclonal Antibody; NT (DACH2; Dachshund homolog 2) (FITC); Dachshund homolog 2; anti-DACH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DACH2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the N-terminal region of human DACH2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DACH2 Antibody (N-term) western blot analysis in HepG2,Jurkat cell line lysates (35ug/lane).This demonstrates the DACH2 antibody detected the DACH2 protein (arrow).)

Product Categories/Family for anti-DACH2 antibody

Antibodies; Transcription Factors

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Styrkarsdottir, U., et al. Nat. Genet. 41(1):15-17(2009). Poulin, F., et al. Genomics 85(6):774-781(2005). Bione, S., et al. Hum. Reprod. 19(12):2759-2766(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 16876441
• NCBI GeneID: 117154
• NCBI Accession #: NP_444511.1; NP_001132987.1
• NCBI GenBank Nucleotide #: NM_053281.3
• UniProt Accession #: Q96NX9; Q8NAY3; Q8ND17; Q96N55; B1AJV3; B4DQG3
• Molecular Weight: 47,156 Da
• NCBI Official Full Name: dachshund homolog 2 isoform a
• NCBI Official Synonym Full Names: dachshund family transcription factor 2
• NCBI Official Symbol: DACH2
• NCBI Protein Information: dachshund homolog 2
• UniProt Protein Name: Dachshund homolog 2
• Protein Family: Dachshund
• UniProt Gene Name: DACH2
• UniProt Synonym Gene Names: Dach2
• UniProt Entry Name: DACH2_HUMAN

NCBI Description

This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

DACH2: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. Belongs to the DACH/dachshund family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq21.3

Cellular Component: nucleus; transcription factor complex

Biological Process: multicellular organismal development; regulation of transcription, DNA-dependent