Rabbit anti-Human, Mouse DACH2 Polyclonal Antibody | anti-DACH2 antibody

DACH2 Rabbit Polyclonal

Cat. Num. AAA768463

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: DACH2; Polyclonal Antibody; DACH2 Rabbit Polyclonal; anti-DACH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 571

• Applicable Applications for anti-DACH2 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Dachshund homolog 2 (Drosophila)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(mouse heart tissue were subjected to SDS PAGE followed by western blot with MBS768463 (DACH2 Antibody) at dilution of 1:600)

NCBI and Uniprot Product Information

• NCBI GI #: 213972566
• NCBI GeneID: 117154
• NCBI Accession #: NP_001132986.1
• NCBI GenBank Nucleotide #: NM_001139514.1
• Molecular Weight: 47,156 Da
• NCBI Official Full Name: dachshund homolog 2 isoform b
• NCBI Official Synonym Full Names: dachshund family transcription factor 2
• NCBI Official Symbol: DACH2
• NCBI Protein Information: dachshund homolog 2
• UniProt Protein Name: Dachshund homolog 2
• Protein Family: Dachshund
• UniProt Gene Name: DACH2
• UniProt Synonym Gene Names: Dach2
• UniProt Entry Name: DACH2_HUMAN

NCBI Description

This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

DACH2: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. Belongs to the DACH/dachshund family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq21.3

Cellular Component: nucleus; transcription factor complex

Molecular Function: DNA binding

Biological Process: development of primary female sexual characteristics; multicellular organismal development; regulation of transcription, DNA-dependent; transcriptional preinitiation complex assembly