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Western Blot (WB) (Western blot analysis of Cytokeratin 16 expression in HaCat cell lysate.)

Rabbit anti-Human Cytokeratin 16 Polyclonal Antibody | anti-CK16 antibody

Anti-Cytokeratin 16 Rabbit pAb

Gene Names
KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
Cytokeratin 16; Polyclonal Antibody; Anti-Cytokeratin 16 Rabbit pAb; CK-16; cytokeratin 16; Cytokeratin-16; FNEPPK; K16; K1C16; K1CP; keratin 16; Keratin-16; KRT16; KRT16A; NEPPK; anti-CK16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytokeratin 16 Antibody detects endogenous levels of total Cytokeratin 16
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
473
Applicable Applications for anti-CK16 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human Cytokeratin 16
Subcellular Location
Mitochondrion membrane; Multi-pass membrane protein
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Cytokeratin 16 expression in HaCat cell lysate.)

Western Blot (WB) (Western blot analysis of Cytokeratin 16 expression in HaCat cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human skin, using Cytokeratin 16 Antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human skin, using Cytokeratin 16 Antibody.)
Related Product Information for anti-CK16 antibody
K16 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8].

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51kDa
NCBI Official Full Name
keratin, type I cytoskeletal 16
NCBI Official Synonym Full Names
keratin 16
NCBI Official Symbol
KRT16
NCBI Official Synonym Symbols
K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
NCBI Protein Information
keratin, type I cytoskeletal 16
UniProt Protein Name
Keratin, type I cytoskeletal 16
UniProt Gene Name
KRT16
UniProt Synonym Gene Names
KRT16A; CK-16; K16

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

Uniprot Description

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Research Articles on CK16

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Product Notes

The CK16 krt16 (Catalog #AAA4750059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 16 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 FC/FACS: 1:50. Researchers should empirically determine the suitability of the CK16 krt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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