Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat Cystatin 8 Polyclonal Antibody | anti-CST8 antibody
Goat anti-Cystatin 8 (aa 100-112) Antibody
Western Blot: In transfected HEK293 transiently expressing Human CST8 (MYC and DYKDDDDK tagged), a band of approx.23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 1-3ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.
Protein type: Cell surface; Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 20p11.21
Cellular Component: cell surface; cytoplasm; extracellular region
Molecular Function: cysteine protease inhibitor activity
Research Articles on CST8
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Product Notes
The CST8 cst8 (Catalog #AAA422291) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cystatin 8 (aa 100-112) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cystatin 8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: In transfected HEK293 transiently expressing Human CST8 (MYC and DYKDDDDK tagged), a band of approx.23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 1-3ug/ml. Researchers should empirically determine the suitability of the CST8 cst8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: STNEICAIQE NSK. It is sometimes possible for the material contained within the vial of "Cystatin 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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