Rabbit FEZ2 Polyclonal Antibody | anti-FEZ2 antibody

FEZ2 Antibody

Cat. Num. AAA151297

• Gene Names: FEZ2; HUM3CL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: FEZ2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: FEZ2; Polyclonal Antibody; FEZ2 Antibody; HUM3CL; Fasciculation and elongation protein zeta-2; Zygin II; fasciculation and elongation protein zeta 2 (zygin II); anti-FEZ2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: FEZ2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-FEZ2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunoflourescence starting at 5 mug/mL. For immunofluorescence start at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: FEZ2 antibody was raised against a 15 amino acid synthetic peptide from near the center of human FEZ2.
• Buffer: FEZ2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FEZ2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of FEZ2 in 3T3 cell lysate with FEZ2 antibody at (A) 0.5, and (B) 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of human brain tissue using FEZ2 antibody at 5 μg/mL.)

Related Product Information for anti-FEZ2 antibody

FEZ2 Antibody: Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 110349756
• NCBI GeneID: 9637
• NCBI Accession #: NP_001036013
• NCBI GenBank Nucleotide #: NM_001042548.1
• UniProt Accession #: Q9UHY8; Q5EBN3; Q76LN0; Q99690
• Molecular Weight: 42,613 Da
• NCBI Official Full Name: fasciculation and elongation protein zeta-2 isoform 2
• NCBI Official Synonym Full Names: fasciculation and elongation protein zeta 2 (zygin II)
• NCBI Official Symbol: FEZ2
• NCBI Official Synonym Symbols: HUM3CL
• NCBI Protein Information: fasciculation and elongation protein zeta-2; zygin 2; zygin-2; zygin II; pre-T/NK cell associated protein (3Cl)
• UniProt Protein Name: Fasciculation and elongation protein zeta-2
• Protein Family: Fasciculation and elongation protein
• UniProt Gene Name: FEZ2
• UniProt Entry Name: FEZ2_HUMAN

NCBI Description

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]

Uniprot Description

FEZ2: Involved in axonal outgrowth and fasciculation. Belongs to the zygin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2p21

Molecular Function: protein binding

Biological Process: axon guidance; nervous system development; signal transduction