CST8 recombinant protein

CST8 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121849

• Gene Names: CST8; CRES; CTES5
• Purity: >85% as determined by SDS-PAGE
• Synonyms: CST8; CST8 Protein; Human; Recombinant (hFc Tag); Human Cystatin-8/CST8 Protein (Fc Tag); CRES Protein; CTES5 Protein; cystatin 8 (cystatin-related epididymal specific); CST8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala142

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CST8 (NP_005483.1)(Met1-Ala142) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4885163
• NCBI GeneID: 10047
• NCBI Accession #: NP_005483.1
• NCBI GenBank Nucleotide #: NM_005492.3
• UniProt Accession #: O60676; Q2M2X6
• Molecular Weight: 16,275 Da
• NCBI Official Full Name: cystatin-8
• NCBI Official Synonym Full Names: cystatin 8
• NCBI Official Symbol: CST8
• NCBI Official Synonym Symbols: CRES; CTES5
• NCBI Protein Information: cystatin-8
• UniProt Protein Name: Cystatin-8
• Protein Family: Cystatin
• UniProt Gene Name: CST8
• UniProt Synonym Gene Names: CRES
• UniProt Entry Name: CST8_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Cell surface; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasm; extracellular region

Molecular Function: cysteine protease inhibitor activity