Rabbit anti-Human Bcl 2 Monoclonal Antibody | anti-Bcl2 antibody

Bcl 2, phosphorylated (Ser70) (B Cell Leukemia 2)

Cat. Num. AAA609596

• Gene Names: Bcl2; Bcl-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Bcl 2; Monoclonal Antibody; phosphorylated (Ser70) (B Cell Leukemia 2); Anti -Bcl 2; anti-Bcl2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7A4
• Specificity: Detects endogenous levels of human Ser70 phosphorylated Bcl 2. It does not crossreact with non-phosphorylated Bcl 2 at endogenous levels or with other Bcl 2 family members.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-Bcl2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Flow Cytometry and Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,; 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1uM, overnight) and with or without phosphatase, using phospho-Bcl-2 (Ser70) Rabbit mAb (upper) or Bcl-2 (lower).; Flow Cytometry: 1:25. Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content).; Immunoprecipitation: 1:50
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser70 of human Bcl 2 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, untreated or|treated with paclitaxel (1uM, overnight) and with or without l|phosphatase, using B0807-04A|(upper) or Bcl-2 (lower).)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells with or without treatment of paclitaxel (1uM, overnight), using MBS609596)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells, using MBS609596 versus propidium iodide (DNA content).)

Related Product Information for anti-Bcl2 antibody

Bcl 2 has the ability to exert a survival function in response to a wide range of apoptotic stimuli by blocking mitochondrial release of cytochrome c (1). It has been implicated in modulating mitochondrial Ca2+ homeostasis and proton flux (2). Several phosphorylation sites have been identified within the Bcl 2 loop domain, including serine 70 (3). It has been suggested that phosphorylation at Ser70 may involve an ASK1/MKK7/JNK1 pathway and may be a marker for mitotic events (4,5). Interleukin 3 and JNK induced Bcl 2 phosphorylation at Ser70 may be required for its enhanced antiapoptotic functions (6).

Product Categories/Family for anti-Bcl2 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 1004379
• NCBI GeneID: 24224
• UniProt Accession #: P49950; Q62837; Q64032; Q7TSN8; Q9R1B3
• Molecular Weight: 26,622 Da
• NCBI Official Full Name: BCL-2
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2
• NCBI Official Symbol: Bcl2
• NCBI Official Synonym Symbols: Bcl-2
• NCBI Protein Information: apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Apoptosis regulator
• UniProt Gene Name: Bcl2
• UniProt Synonym Gene Names: Bcl-2
• UniProt Entry Name: BCL2_RAT

NCBI Description

an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD]

Uniprot Description

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Membrane protein, integral; Autophagy; Oncoprotein

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; mitochondrion; endoplasmic reticulum; cell; integral to membrane; cytosol; pore complex; mitochondrial outer membrane; membrane; cytoplasm; mitochondrial membrane; intracellular; nucleus; myelin sheath

Molecular Function: identical protein binding; BH domain binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; channel activity; channel inhibitor activity; protein phosphatase binding; transcription factor binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding; BH3 domain binding

Biological Process: positive regulation of catalytic activity; focal adhesion formation; developmental growth; pigment granule organization and biogenesis; regulation of cell cycle; response to toxin; ear development; negative regulation of myeloid cell apoptosis; B cell receptor signaling pathway; regulation of protein localization; negative regulation of ossification; oligodendrocyte differentiation; regulation of transmembrane transporter activity; negative regulation of neuron apoptosis; kidney development; T cell differentiation; response to drug; spleen development; positive regulation of neuron maturation; axon regeneration; cell aging; regulation of calcium ion transport; positive regulation of cell growth; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; regulation of gene expression; response to heat; negative regulation of osteoblast proliferation; response to oxidative stress; lymphocyte homeostasis; negative regulation of apoptosis; positive regulation of pigmentation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to insulin stimulus; response to organic substance; negative regulation of cell proliferation; positive regulation of skeletal muscle fiber development; ureteric bud development; positive regulation of cell proliferation; negative regulation of cell migration; transmembrane transport; regulation of viral genome replication; response to nutrient; leukocyte homeostasis; aging; mesenchymal cell development; response to aluminum ion; CD8-positive, alpha-beta T cell lineage commitment; response to inorganic substance; B cell lineage commitment; positive regulation of peptidyl-serine phosphorylation; response to UV-B; cell proliferation; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; response to copper ion; pigmentation; ureteric bud branching; homeostasis of number of cells within a tissue; response to hypoxia; negative regulation of cell growth; brain development; response to nicotine; renal system process; protein polyubiquitination; response to glucocorticoid stimulus; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; response to organic cyclic substance; glomerulus development; post-embryonic development; response to caffeine; cochlear nucleus development; regulation of apoptosis; cellular response to glucose starvation; cell-cell adhesion; regulation of mitochondrial membrane potential; response to folic acid; positive regulation of B cell proliferation; T cell homeostasis; negative regulation of mitotic cell cycle; cell growth; response to electrical stimulus; defense response to virus; response to corticosterone stimulus; release of cytochrome c from mitochondria; regulation of protein homodimerization activity; actin filament organization; digestive tract morphogenesis; organ growth; organ morphogenesis; induction of apoptosis via death domain receptors; response to steroid hormone stimulus; B cell differentiation; gland morphogenesis; regulation of pigmentation during development; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; B cell proliferation; response to peptide hormone stimulus; regulation of protein heterodimerization activity; response to cAMP; behavioral fear response; melanin metabolic process; negative regulation of retinal cell programmed cell death; apoptosis; regulation of cell-matrix adhesion; pigmentation during development; cell morphogenesis; regulation of protein stability; protein amino acid dephosphorylation; apoptotic mitochondrial changes; ovarian follicle development; B cell homeostasis; positive regulation of melanocyte differentiation; regulation of catalytic activity; melanocyte differentiation; response to gamma radiation; hemopoiesis; negative regulation of cellular pH reduction; response to iron ion; ossification; hair follicle morphogenesis; thymus development; male gonad development; peptidyl-threonine phosphorylation; regulation of programmed cell death; cellular calcium ion homeostasis; immune system development; T cell lineage commitment; neuron apoptosis; axonogenesis; response to hydrogen peroxide; response to cytokine stimulus; induction of apoptosis by oxidative stress; response to DNA damage stimulus; growth