Western Blot (WB)
(Western blot analysis of CCND1 (arrow) using rabbit polyclonal CCND1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCND1 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human Cyclin D1 Polyclonal Antibody | anti-cyclin D1 antibody
Cyclin D1, ID (CCND1 Protein, Cyclin D1, Isoform CRA_c, cDNA, FLJ93625, Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1),mRNA, Cyclin D1, PRAD1: Parathyroid Adenomatosis 1, CCND1)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Cyclin D1; Polyclonal Antibody; ID (CCND1 Protein; Isoform CRA_c; cDNA; FLJ93625; Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1); mRNA; PRAD1: Parathyroid Adenomatosis 1; CCND1); Anti -Cyclin D1; anti-cyclin D1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CCND1.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-cyclin D1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human CCND1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CCND1 (arrow) using rabbit polyclonal CCND1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCND1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of CCND1 (arrow) using rabbit polyclonal CCND1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCND1 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-cyclin D1 antibody
CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Product Categories/Family for anti-cyclin D1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Cyclin D1 cyclin d1 (Catalog #AAA625110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclin D1, ID (CCND1 Protein, Cyclin D1, Isoform CRA_c, cDNA, FLJ93625, Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1),mRNA, Cyclin D1, PRAD1: Parathyroid Adenomatosis 1, CCND1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin D1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the Cyclin D1 cyclin d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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