Western Blot (WB)
(Western blot analysis of GABRA4 Antibody (Center) in CEM cell line lysates (35ug/lane). GABRA4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human GABRA4 Polyclonal Antibody | anti-GABRA4 antibody
GABRA4, ID (GABRA4, Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of GABRA4 Antibody (Center) in CEM cell line lysates (35ug/lane). GABRA4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GABRA4 Antibody (Center) in CEM cell line lysates (35ug/lane). GABRA4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(GABRA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GABRA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GABRA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GABRA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GABRA4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GABRA4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011]
Uniprot Description
GABRA4: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily.
Protein type: Membrane protein, multi-pass; Transporter, ion channel; Transporter; Channel, chloride; Channel, ligand-gated; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4p12
Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction
Molecular Function: chloride channel activity; GABA-A receptor activity; benzodiazepine receptor activity; extracellular ligand-gated ion channel activity
Biological Process: synaptic transmission; central nervous system development; transport; transmembrane transport; gamma-aminobutyric acid signaling pathway
Research Articles on GABRA4
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Product Notes
The GABRA4 gabra4 (Catalog #AAA6300852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABRA4, ID (GABRA4, Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GABRA4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GABRA4 gabra4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABRA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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