Western Blot (WB)
(Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa))
Mouse anti-Human TWF1 Monoclonal Antibody | anti-TWF1 antibody
TWF1 Antibody
Gene Names
TWF1; A6; PTK9
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
TWF1; Monoclonal Antibody; TWF1 Antibody; A6; A6 protein tyrosine kinase; MGC105817; MGC23788; MGC41876; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; twf1; TWF1_HUMAN; Twinfilin 1; Twinfilin actin binding protein; homolog 1; Twinfilin; actin-binding protein; homolog 1 (Drosophila); Drosophila; homolog of; 1; Twinfilin-1; anti-TWF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
TWF1 antibody detects endogenous levels of total TWF1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
357
Applicable Applications for anti-TWF1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human TWF1 expressed in E. Coli
Subcellular Location
Cytoplasm. Cytoplasm > Cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
Tissue Specificity
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa))
Related Product Information for anti-TWF1 antibody
Description: This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Subunit Structure: Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4, 5)P2. When bound to PI(4, 5)P2, it is down-regulated (By similarity). Interacts with ACTG1 (PubMed:28493397).
Post-translational Modifications: Phosphorylated on serine and threonine residues.
Similarity: Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Subunit Structure: Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4, 5)P2. When bound to PI(4, 5)P2, it is down-regulated (By similarity). Interacts with ACTG1 (PubMed:28493397).
Post-translational Modifications: Phosphorylated on serine and threonine residues.
Similarity: Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 40.3 kDa
Predicted: 41 kDa
Predicted: 41 kDa
NCBI Official Full Name
twinfilin-1 isoform 1
NCBI Official Synonym Full Names
twinfilin actin binding protein 1
NCBI Official Symbol
TWF1
NCBI Official Synonym Symbols
A6; PTK9
NCBI Protein Information
twinfilin-1
UniProt Protein Name
Twinfilin-1
Protein Family
UniProt Gene Name
TWF1
UniProt Synonym Gene Names
PTK9
NCBI Description
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]
Uniprot Description
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles ().
Research Articles on TWF1
Similar Products
Product Notes
The TWF1 twf1 (Catalog #AAA9602321) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TWF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TWF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the TWF1 twf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
