Rabbit anti-Human, Mouse TLR4 Polyclonal Antibody | anti-TLR4 antibody

TLR4 Antibody

Cat. Num. AAA805758

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Western Blot
• Synonyms: TLR4; Polyclonal Antibody; TLR4 Antibody; ARMD10; CD284; TLR 4; TOLL; Toll like receptor 4; anti-TLR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 839

• Applicable Applications for anti-TLR4 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: 1-3ug/mL (WB), 1:50 (IHC)
• Certificate of Analysis: 2 ug/mL of SPC-200 was sufficient for detection of TLR4 in 100ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Conjugate: Unconjugated
• Storage Buffer: PBS, 0.05% BSA, 0.05% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.)

Western Blot (WB)

(Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)

Related Product Information for anti-TLR4 antibody

Background Info: Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Scientific Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).

Product Categories/Family for anti-TLR4 antibody

Cell Signaling; Organelle Markers

References

1.JMuzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997). Cell 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta 1518 (1-2): 157-161). 4. Takeuchi O., et al. (1999) Immunity 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature 388 (6640): 394-397.

NCBI and Uniprot Product Information

• NCBI GI #: 19924149
• NCBI GeneID: 7099
• NCBI Accession #: NP_612564.1
• NCBI GenBank Nucleotide #: NM_138554.4
• UniProt Accession #: O00206; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53
• Molecular Weight: 73,301 Da
• NCBI Official Full Name: toll-like receptor 4 isoform A
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4; hToll; homolog of Drosophila toll
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR4
• UniProt Entry Name: TLR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: integral to plasma membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; endosome membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; negative regulation of interleukin-6 production; positive regulation of interferon-beta production; positive regulation of B cell proliferation; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; T-helper 1 type immune response; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; macrophage activation; negative regulation of interleukin-23 production; detection of lipopolysaccharide; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of osteoclast differentiation; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of JNK cascade; defense response to Gram-negative bacterium; positive regulation of interleukin-8 production; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; lipopolysaccharide-mediated signaling pathway; interferon-gamma production; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; detection of fungus; MyD88-independent toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of interleukin-1 production; positive regulation of inflammatory response

Disease: Macular Degeneration, Age-related, 10