Rabbit Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody

Rabbit anti Cyclin B1 Polyclonal Antibody

Cat. Num. AAA460204

• Gene Names: CCNB1; CCNB
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Cyclin B1; Polyclonal Antibody; Rabbit anti Cyclin B1 Polyclonal Antibody; CCNB; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes 62 kDa of Cyclin B1 Protein. It reacts with human, mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 113

• Applicable Applications for anti-CCNB1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide derived from C-terminus of human cyclin B1 protein.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-CCNB1 antibody

Cyclin B1 is a regulatory protein involved in mitosis. Like all cyclins, levels of cyclin B1 oscillate over the course of the cell cycle. Just prior to mitosis, a large amount of cyclin B1 is present in the cell, but it is inactive due to phosphorylation of Cdk1 by the Wee1 kinase. The complex is activated by dephosphorylation by the phosphatase Cdc25. Once cyclin B1-Cdk1 is activated, it remains stably active for the rest of mitosis. At the end of mitosis, cyclin B1 is targeted for degradation by the APC through its APC localization sequence permitting to exit mitosis. Cyclin B1/Cdc2 complexes, which appear to be constitutively cytoplasmic during interphase, actually shuttle continually into and out of the nucleus, with the rate of nuclear export exceeding the import rate. Cell growth prior to cell division is restricted by the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complexes. Cdk1/cyclin B1 activity shields human cells against extrinsic death stimuli and unravel the molecular details of the cross talk between cell cycle and extrinsic apoptotic pathways

References

Miyazaki, T. Arai, S. Two distinct controls of mitotic cdk1/cyclin B1 activity requisite for cell growth prior to cell division. Cell Cycle. 6 (12):1419-25 (2007). Berry LD, Gould KL. "Regulation of Cdc2 activity by phosphorylation at T14/Y15". Prog Cell Cycle Res 2: 99 -105 (1996).Yang J, Song H, Walsh S, Bardes ES, Kornbluth S."Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites". J. Biol. Chem. 276 (5): 3604 -9 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13518072
• NCBI GeneID: 891
• Molecular Weight: 44,102 Da
• NCBI Official Full Name: cyclin B1, partial
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB1
• UniProt Synonym Gene Names: CCNB

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: centrosome; cytoplasm; cytosol; mitochondrial matrix; nucleoplasm; nucleus; spindle pole

Molecular Function: cyclin-dependent protein kinase activity; patched binding; protein binding; protein kinase binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G2/M transition of mitotic cell cycle; G2/M-specific positive regulation of cyclin-dependent protein kinase activity; mitotic metaphase plate congression; mitotic nuclear envelope disassembly; mitotic spindle organization and biogenesis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle