Immunoprecipitation (IP)
(CCNA1 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate0.5 uL anti-CCNA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCNA1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
Rabbit anti-Human Cyclin A1 Polyclonal Antibody | anti-CCNA1 antibody
Anti-Cyclin A1 Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
IP: 1-2uL/mg of lysate
Immunoprecipitation (IP)
(CCNA1 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate0.5 uL anti-CCNA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCNA1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
Immunoprecipitation (IP)
(CCNA1 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate0.5 uL anti-CCNA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCNA1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
Western Blot (WB)
(Anti-CCNA1 rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:65 kDaObserved band size:85 kDa)
Western Blot (WB)
(Anti-CCNA1 rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:65 kDaObserved band size:85 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Cancer Testis Antigen (CTA); Cell cycle regulation
Chromosomal Location of Human Ortholog: 13q12.3-q13
Cellular Component: nucleoplasm; microtubule cytoskeleton; cytosol
Molecular Function: protein binding; protein kinase binding
Biological Process: G1/S-specific transcription in mitotic cell cycle; mitosis; male meiosis I; cell division; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle
Research Articles on CCNA1
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Product Notes
The CCNA1 ccna1 (Catalog #AAA8104572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin A1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the CCNA1 ccna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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