Rabbit Cyclin A1 Polyclonal Antibody | anti-CCNA1 antibody

Anti-Cyclin A1 Antibody

Cat. Num. AAA416819

• Gene Names: CCNA1; CT146
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Protein A purified
• Synonyms: Cyclin A1; Polyclonal Antibody; Anti-Cyclin A1 Antibody; anti-CCNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cyclin A1 antibody detects endogenous levels of Cyclin A1 protein. Not tested with other proteins
• Purity/Purification: Protein A purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 411

• Applicable Applications for anti-CCNA1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:20,000; WB: 1:200 - 1:1000; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-CCNA1 antibody

This gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

References

Gong, J., U. Bhatia, F. Traganos and Z. Darzynkiewicz. Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry

NCBI and Uniprot Product Information

• NCBI GI #: 2183079
• NCBI GeneID: 8900
• Molecular Weight: 47,494 Da
• NCBI Official Full Name: cyclin A1, partial
• NCBI Official Synonym Full Names: cyclin A1
• NCBI Official Symbol: CCNA1
• NCBI Official Synonym Symbols: CT146
• NCBI Protein Information: cyclin-A1
• UniProt Protein Name: Cyclin-A1
• Protein Family: Cyclin
• UniProt Gene Name: CCNA1
• UniProt Entry Name: CCNA1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12.3-q13

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; mitosis; male meiosis I; cell division; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle