BLOC1S2 recombinant protein

BLOC1S2 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121580

• Gene Names: BLOC1S2; CEAP; BLOS2; CEAP11
• Purity: >74% as determined by SDS-PAGE
• Synonyms: BLOC1S2; BLOC1S2 Protein; Human; Recombinant (GST Tag); Human BLOC1S2/BLOS2 Protein (GST Tag); BLOS2 Protein; CEAP Protein; CEAP11 Protein; biogenesis of lysosomal organelles complex-1; subunit 2; BLOC1S2 recombinant protein

• Host: E Coli
• Purity/Purification: >74% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg99

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human BLOC1S2 (Q6QNY1-2)(Met1-Arg99) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BLOC1S2 recombinant protein

Background: BLOC1S2, also known as BLOS2, belongs to the BLOC1S2 family. It is a component of BLOC-1 complex. The BLOC-1 complex is composed of BLOC1S1, BLOC1S2, BLOC1S3, DTNBP1, MUTED, PLDN, CNO/cappuccino and SNAPIN. The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. BLOC1S2 interacts directly with BLOC1S1, BLOC1S3, MUTED, CNO/cappuccino and SNAPIN. It may play a role in cell proliferation. It also plays a role in intracellular vesicle trafficking. Functionally, BLOC1S2 gene has been proposed to participate in processes (melanosome organization, microtubule nucleation, platelet dense granule organization, positive regulation of cell proliferation, positive regulation of transcription, regulation of apoptosis, positive regulation of transcription from RNA polymerase II promoter).

References

Sowa ME, et al. (2009) Defining the human deubiquitinating enzyme interaction landscape. Cell. 138(2):389-403.Gdynia G, et al. (2008) BLOC1S2 interacts with the HIPPI protein and sensitizes NCH89 glioblastoma cells to apoptosis. Apoptosis. 13(3):437-47.Starcevic M, et al. (2004) Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). J Biol Chem. 279(27):28393-401.

NCBI and Uniprot Product Information

• NCBI GI #: 47717129
• NCBI GeneID: 282991
• NCBI Accession #: NP_001001342.1
• NCBI GenBank Nucleotide #: NM_001001342.2
• UniProt Accession #: Q6QNY1; Q5W040; Q8WUI8; B4DQV2
• Molecular Weight: 11,489 Da
• NCBI Official Full Name: biogenesis of lysosome-related organelles complex 1 subunit 2 isoform 2
• NCBI Official Synonym Full Names: biogenesis of lysosomal organelles complex-1, subunit 2
• NCBI Official Symbol: BLOC1S2
• NCBI Official Synonym Symbols: CEAP; BLOS2; CEAP11
• NCBI Protein Information: biogenesis of lysosome-related organelles complex 1 subunit 2; 11 kDa centrosome associated protein; BLOC-1 subunit 2; centrosomal 10 kDa protein; centrosome protein oncogene
• UniProt Protein Name: Biogenesis of lysosome-related organelles complex 1 subunit 2
• Protein Family: Biogenesis of lysosome-related organelles complex
• UniProt Gene Name: BLOC1S2
• UniProt Synonym Gene Names: BLOS2; CEAP; BLOC-1 subunit 2
• UniProt Entry Name: BL1S2_HUMAN

NCBI Description

This gene encodes a protein with multiple functions. The encoded protein has been found in association with the centrosome, shown to co-localize with gamma-tubulin, and also found to be one of the proteins in the BLOC-1 complex which functions in the formation of lysosome-related organelles. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

BLOC1S2: May play a role in cell proliferation. The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Belongs to the BLOC1S2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: centrosome; recycling endosome; mitochondrion; nucleus; gamma-tubulin complex

Molecular Function: protein C-terminus binding; protein binding; gamma-tubulin binding

Biological Process: induction of apoptosis via death domain receptors; platelet dense granule organization and biogenesis; melanosome organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; anterograde synaptic vesicle transport; positive regulation of transcription from RNA polymerase II promoter; microtubule nucleation; anterograde axon cargo transport; neurite development