Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry using Rabbit Anti-Cyclin A antibody. Tissue:human testes tissue. Fixation: formalin fixed paraffin embedded.Antigen retrieval: not required.Primary antibody: Anti-Cyclin A at 1:500 for 1 hr at RT. Localization: Cyclin A is nuclear and cytoplasmic.Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.)
Rabbit Cyclin A Polyclonal Antibody
Anti-Cyclin A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Affinity chromatography
Synonyms
Cyclin A; Polyclonal Antibody; Anti-Cyclin A; anti-Cyclin A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the Cyclin A protein
Purity/Purification
Affinity chromatography
Form/Format
0.01% (w/v) Sodium Azide
Applicable Applications for anti-Cyclin A antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Immunogen
Anti-Cyclin A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry using Rabbit Anti-Cyclin A antibody. Tissue:human testes tissue. Fixation: formalin fixed paraffin embedded.Antigen retrieval: not required.Primary antibody: Anti-Cyclin A at 1:500 for 1 hr at RT. Localization: Cyclin A is nuclear and cytoplasmic.Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry using Rabbit Anti-Cyclin A antibody. Tissue:human testes tissue. Fixation: formalin fixed paraffin embedded.Antigen retrieval: not required.Primary antibody: Anti-Cyclin A at 1:500 for 1 hr at RT. Localization: Cyclin A is nuclear and cytoplasmic.Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.)
Western Blot (WB)
(Sample Data: Western Blot of Rabbit Anti-Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr).Lane 3: T98G cells treated with PIN1 knockdown (kd1).Load: 25 ug per lane. Primary antibody: Anti-Cyclin A at 1:400 for overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.)
Western Blot (WB)
(Sample Data: Western Blot of Rabbit Anti-Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr).Lane 3: T98G cells treated with PIN1 knockdown (kd1).Load: 25 ug per lane. Primary antibody: Anti-Cyclin A at 1:400 for overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.)
Related Product Information for anti-Cyclin A antibody
Anti-Cyclin A detects cyclin a. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase CDK2 and is required for the cell to progress through the S phase. CDK2/Cyclin A is inhibited by the complex p21CIP. The Anti-Cyclin-A antibody is ideal for investigators involved in cell signaling, cell biology and signal transduction research.
Product Categories/Family for anti-Cyclin A antibody
References
1. Rizzolio F, Lucchetti C, et al, Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation 1-10. Nature 2012.
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Product Notes
The Cyclin A (Catalog #AAA515920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin A reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Cyclin A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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