Rabbit anti-Human, Mouse Cyclin E1 Polyclonal Antibody | anti-CCNE1 antibody

Cyclin E1 (G1/S-specific Cyclin-E1, CCNE1, CCNE) (AP)

Cat. Num. AAA6375314

• Gene Names: CCNE1; CCNE
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cyclin E1; Polyclonal Antibody; Cyclin E1 (G1/S-specific Cyclin-E1; CCNE1; CCNE) (AP); anti-CCNE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCNE1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CCNE1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CCNE1, aa1-410 (NP_001229.1).
• Immunogen Sequence: MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CCNE1 expression in transfected 293T cell line by CCNE1 polyclonal antibody. Lane 1: CCNE1 transfected lysate (47.1kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between CCNE1 and CDKN1A. HeLa cells were stained with CCNE1 rabbit purified polyclonal 1:1200 and CDKN1A mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-CCNE1 antibody

Regulated progression through the cell cycle requires sequential expression of a family of proteins called cyclins. Upon their induction, cyclins form complexes with specific cyclin-dependent kinases (CDKs), creating active holoenzymes that phosphorylate target proteins that are required for cell-cycle progression. In higher eukaryotes, CDKl associated with mitotic cyclins (cyclin A, B1, and B2) controls the G2/M transition, while multiple CDK-cyclin complexes control the GUS transition. Putative G1 cyclins (cyclin C, D, and E) were isolated when human and Drosophila cDNA libraries were used to complement yeast G1 mutants. Among the human G1 cyclins, only cyclin E was shown to fluctuate periodically through the cell cycle. The amounts of cyclin E mRNA, protein, and CDK2-associated protein kinase activity were maximal in late G1 in HeLa cells. Functional analysis suggested that cyclin E is rate-limiting for G1 progression, since overexpression of cyclin E accelerated the GUS transition in mammalian fibroblasts, while microinjection of anti-cyclin E antibodies inhibited this transition. In addition, cyclin E was shown to be required for S phase entry and to specifically induce transcription of S phase genes (DNA polymerase a, PCNA, and two ribonucleotide reductase subunits) in Drosophila. It was also reported that multiple levels of regulation of the cyclin E mRNA, protein, and associated kinase activity are present during the first 24h of zebrafish embryonic development.

Product Categories/Family for anti-CCNE1 antibody

Antibodies; Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 17318559
• NCBI GeneID: 898
• NCBI Accession #: NP_001229.1
• NCBI GenBank Nucleotide #: NM_001238
• UniProt Accession #: P24864; Q14091; Q8NFG1; Q92501; Q9UD21; A8K684
• Molecular Weight: 47,077 Da
• NCBI Official Full Name: G1/S-specific cyclin-E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin Es; cyclin Et
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE
• UniProt Entry Name: CCNE1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle