Rabbit anti-Human, Mouse CXCR4 Polyclonal Antibody | anti-CXCR4 antibody

CXCR4, NT (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184) (Azide Free)

Cat. Num. AAA6471096

• Reactivity: Human, Mouse
• Applications: Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Purified IgG
• Synonyms: CXCR4; Polyclonal Antibody; NT (Fusin; LESTR; HUMSTR; C-X-C Chemokine Receptor 4; CD184) (Azide Free); anti-CXCR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CXCR4. Species crossreactivity: mouse.
• Purity/Purification: Purified IgG
• Form/Format: Supplied as a liquid in PBS. No preservative added.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CXCR4 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC: 10ug/ml; WB: 1-2ug/ml; IP: 10ug/ml
• Immunogen: Synthetic peptide corresponding to aa1-14 of human CXCR4.
• Positive Control: HeLa whole cell lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CXCR4 in HeLa whole cell lysate with MBS6471096 at 1:2000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of C8375)

Related Product Information for anti-CXCR4 antibody

HIV and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The alpha-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (10,11).

Product Categories/Family for anti-CXCR4 antibody

Antibodies; Growth Factors; Cytokines

References

1. Dimitrov, D, S. Cell (1997). 91:721-730 2. Feng, Y. et al. Science (1996). 272:872-7. 3. Berson, J.F. et al. J Virol (1996). 70:6288-6295. 4. Doranz, B.J. et al. Cell (1996). 85:1149-1158 5. Endres, M.J. et al. Cell (1996). 87:745-756 6. Bleul, C.C. et al. Nature (1996). 382:829-833 7. Oberlin, E. et al. Nature (1996). 382:833-835 8. Lapham, C.K. et al. Science (1996). 274:602-605. 9. Leoetscher, M. et al. J Biol Chem (1994) 269:232-237 10. Brelot, A. et al. J Virol (1997)71:4744-4751. 11. Lu, Z. et al. Proc Natl Acad Sci USA (1997). 94:6426-6431.